Citopathology II

Base Knowledge

Anatomy, Histology and Histotecnology

Teaching Methodologies

 The theoretical classes are aimed at the detailed presentation of the contents. Use an expository -active methodology, lectures, presentations with discussion and debate.

The practical part of curricular unit focuses on the view of blades, as well as the elaboration of a cytopathological report.

Learning Results

 

 

Differentiate the procedures of a technical nature for different types of cytological material.
– To identify cell patterns for respiratory cytology, breast aspiration cytology, thyroid aspiration cytology, ganglion  aspiration cytology.

– Know the application of immunocytochemistry, flow cytometry and molecular biology techniques in exfoliative cytology and aspiration puncture.

– Carry out the screening with the interpretation and preparation of the respective report.

 

Program

RESPIRATORY CYTOLOGY

Introduction to cytological technique and normal cytology in respiratory specimens

Inflammatory, infectious and non neoplastic changes

Lung Neoplasias

Iatrogenic changes

Cytopathological report

New Ratings

ASPIRATION CYTOLOGY

Technical procedures on FNA

Aspiration punctures ultrasound guided and ecoendoscopy

BREAST ASPIRATION CYTOLOGY

Normal cytology

Inflammatory changes

Benign and malignant primary pathology

Metastatic tumours

New Ratings

ASPIRATION CYTOLOGY OF THYROID

Normal cytology

Inflammatory changes

Benign pathology and primary and secondary neoplastic

Bethesda classification

ASPIRATION CYTOLOGY OF LYMPH NODE

Normal and reactive inflammatory cytology

Primary and metastatic neoplastic pathology

importance of techniques in immunocytochemistry, flow cytometry and molecular biology in cytopathological diagnosis.

 

PRACTICE

– Understanding of the pre analytical phase. Concepts and examples:

– Collection and transport of samples

– Requirements and registrations. Care to be taken.

– Execution of smears in exfoliative cytology.

– Simulation of aspiration puncture collection and swab execution.

-Staining Techniques: Papanicolaou, Giemsa and Hematoxylin Eosin.

-Execution of imprint.

-Execution of conventional “Cell Block”.

– Execution of the “Cell Block” from the remaining material of cytology in liquid  based.

-Microscope evaluation for quality control

-Screening of the theoretical material samples: lung, thyroid, breast and lymph node.

 

Curricular Unit Teachers

Internship(s)

NAO

Bibliography

Primary References:

1. Cibas, E.D. (2010). The Bethesda System for Reporting Thyroid Cytopathology- definitions, criteria and explanatory notes

2. Mckee, G.T. (1997). Cytopathology. London:Mosby-Wolfe

3. Koss, L.G. (2006). Diagnostic Cytology and its histopathologic bases.5th Edition.

Philadelphia:J.B.Lippincott.Vol.I e II

4. Keebler C. M. Somrak T.M. (1993). The manual of Cytophatology. 7th Edition.USA: ASCP

5. Kurman R.J., Solomon D. (2004). THE BETHESDA SYSTEM for reporting cervical/vaginal cytology

diagnosis – definitions, criteria and explanatory notes for terminology and specimen adequacy. New York, Inc. Springer – Verlag

6. Wield, G. L. Keebler C.M., KossL. G., Patten S. F., Rosenthal D.L. (1992). Compendium on Diagnostic Cytology. 7th Edition. Chicago, Illinois, USA: Tutorial of Cytology

 

Secondary References:

Atkinson B.F., Silverman J.F. (2000) Atlas de dificuldades diagnósticas em citopatologia:Madrid:Harcout