Culture of Animal Cells

Base Knowledge

Biology I
Introduction to biotechnology
Biology II
Anatomy, phyisiology and histology animal

Teaching Methodologies

In order to achieve the objectives of the curricular unit and the acquisition of the respective competences by the students, the teaching and learning process is based on the following teaching methodologies:
a) Lectures of theoretical / practical classes;
b) Execution of practical works of cell culture in the laboratory and in the classroom by simulation.
c) Bibliographic research, article analysis and presentation of papers.
Students are required to attend classes. The minimum attendance value is 75% of the number of classes taught.

Learning Results

Animal cell culture is an important technique in many molecular biology, development biology and biotechnology laboratories. It is a technique that is becoming increasingly important in research in the field of biology, tissue replacement therapy, therapeutic proteins and others. This technique allows animal tissue models to be constructed and used as alternatives to experimentation on live animals
The learning objectives of this UC are intended to provide the student who has successfully completed the following competencies:
1) Have understanding of animal cell culture techniques and the functioning standards of a cell culture laboratory.
2) To know the constituents of the culture medium and strategies of formulation of culture media for animal cells.
3) Understand the aseptic techniques of manipulation and maintenance in the culture of animal cells
4) To know the applications of cell culture techniques in animal health and breeding.

Program

Module 1 (50%): Introduction to Animal Cell Culture
Introduction – History of Animal Cell Culture (CCA). Advantages and limitations. Types of tissues used in cell culture. Applications of the technique.
Ideal environment for the culture of animal cells: conditions and equipments required; laboratory.design
Mediums necessary for the culture of animal cells.
Classification and basic characteristics of cell culture: culture of primary cells and secondary cells; organotypic and histotypical cell cultures.
Growth and maintenance of cells in culture (Practical classes)
Module 2: (50%)
Contamination of Cell Cultures: types of contamination, their identification and control. How to solve the problems.
Selection of tissue types for cell culture: tissue of adult or embryonic origin; stem cells; normal or tumor tissue.
Cell differentiation: transformation and immortalization of cells.
Cryopreservation of cells
Culture of Specialized Cells: epithelial, mesenchymal, neuronal, hematopoietic, gonad

Curricular Unit Teachers

Grading Methods

Avaliação Por Exame
  • - Componente teórico (escrita) 75% + Componente prática (escrita ou laboratório) 25%. Ambos os módulos têm uma ponderação de 50%. - 100.0%
Avaliação Contínua
  • - CF = (40% nota do teste módulo 1+ 40% nota do teste módulo 2 + 20% nota do trabalho) - 100.0%

Internship(s)

NAO

Bibliography

CLYNES Martin, Animal cell culture techniques, Publisher: Springer Berlin Heidelberg, 1998, ISBN: 978-3-540-63008-1; DOI: 10.1007/978-3-642-80412-0

LANZA Robert, Essentials of Stem Cell Biology, 2nd Edition, Elsevier Academic Press, 2009, ISBN: 978-0-12-374729-7

FRESHNEY R. Ian. Culture of Animal Cells: A Manual of Basic Technique, 5th ed., Published by John Wiley & sons Inc, Hoboken, New Jersy USA. 2005. ISBN-13 978-0-471-45329-1

BUTLER Michael, Animal Cell Culture and Technology, 2nd Edition, Garland Science Publishers: New York, 2003, ISBN: 9781859960493