Teaching Methodologies
The theoretical classes are aimed at the detailed presentation of the program contents. Use an expository active methodology, appealing the direct intervention of the students, favouring openly an abundant iconography instead of the detriment of a theoretical-descriptive teaching.
In the practical part of curricular unit its privileged the execution of practical work in laboratory, explanations and demonstrations using software, multimedia display and resolution of exercises.
The evaluation of theoretical component will include a written assessment (40%) and the presentation of a group project on a topic covered in the program contents (30%). The practical component will be evaluated by a written laboratory report (30%).
Learning Results
To know the theoretical fundaments underlying the instrumental methods discussed, as well as the function of the main components of the instruments used, limitations and potentialities;
Know the principles, methods and techniques related to eletrophoresis and chromatography;
Understand the biomedical applications underlying the electrophoretic and chromatographic methods.
Develop skills in terms of teamwork;
Understand the fundamental concepts of analytical methods studied in order to be able to draw analytical qualitative and/or quantitative conclusions.
Be able to perform eletrophoresis and chromatography protocols;
Select the appropriate methodology to solve a problem of laboratory analysis, taking into account specific requirements as sensitivity, specificity and reproducibility among others;
Critically interpret the results and evaluate their quality.
Program
Chromatographic and electrophoretic separation methods:
General principles, generic applications and biomedical applications; SDS-PAGE electrophoresis;
Two-dimensional electrophoresis (2DE); Capillary electrophoresis;
Western blotting;
Thin layer chromatography; Column chromatography; Hyphenated techniques
Internship(s)
NAO
Bibliography
Skoog, D.A., F.J. Holler, and S.R. Crouch. (2018). Principles of instrumental analysis. Seventh edition. ed. Australia: Cengage Learning.
Stafford, R. (2020) Chromatography: Principles and Instrumentations. NY Research Press.
Grumbach, E. S., J. C. Arsenault, et al. (2014). Beginners guide to UPLC: Ultraperformance liquid chromatography. Milford, MA, Waters.
Meyer, V. (2013). Practical high-performance liquid chromatography. Chichester, U.K., Wiley. Poole, C. F. (2012). Gas chromatography. Amsterdam; Boston, Elsevier.
Sparkman, O. D., Z. Penton, et al. (2011). Gas chromatography and mass spectrometry practical guide., Boston, Elsevier.
Westermeier, R. (2016). Electrophoresis in Practice, fifth edition, Wiley.