Teaching Methodologies
This module will be delivered as a series of three-four hour long workshops (as appropriate) which will be based on pre-lecture material (texts, screen-casts and recorded lectures) made available to the students
ahead of the workshop. Each workshop will be thematic and deal with one particular aspect and the ethos of each workshop will be on the development of analytical skills and knowledge. The module is assessed completely by continuous assessment.
Learning Results
Explain in detail the principle of various forms of bright field and fluorescent microscopy
Compare and contrast different forms for imaging techniques
Design an imaging-based approach for the investigation of a specific biomarker
Explain the essential quality control measures as applied to imaging analysis
Analyse imaging data using appropriate methodologies
Prepare and analyse samples for various forms of microscopy
Program
The module will be dynamic and respond to the continuing developments in imaging technology in order to ensure that the content is aligned with the cutting edge in the field. Typical aspects which form the
foundation of the module will include:
– Principles of microscopy
– Principles of imaging methods for analysis of cell and tissue constituents (including immunohistochemistry, immunofluorescence, in situ hybridisation, confocal microscopy, live cell imaging,
raman microspectroscopy)
– Optimisation of immunostaining techniques
– Specimen dissection and preparation for analysis
– Laser capture microdissection
– Construction of a reporter protein system for cellular analysis
– Image capture systems and image analysis
– Indicative practical components will include: histochemical analysis of cell phenotype, immunocytochemical staining, immunofluorescence, quality control of microscopy
Internship(s)
NAO
Bibliography
Hannon-Fletcher M. and Maxwell P., 2009, Advanced Techniques in Cellular Pathology, Wiley-Blackwell. Dabbs D.J., 2017, Diagnostic Immunohistochemistry: Theranostic and Genomic Applications, Saunders.
Murphy, D.B., 2012, Fundamentals of Light microscopy & Electronic Imaging, Wiley-Blackwell
Watkins, S. and St. Croix, C., 2013, Current Protocols Select: Methods and Applications in Microscopy & Imaging, Wiley
Yuste, R., 2010, Imaging, A Laboratory Manual, Cold Spring Harbour Laboratory Press