Genetic Engineering

Teaching Methodologies

Module 1 Gene manipulation, transformation and PCR applications
Module 2 Modified Organisms
Theoretical and practical classes and reports related to different subjects
For a better consolidation of concepts, students are followed weekly in tutorial tutorial classes.
Students are required to attend classes. The minimum attendance value for each type of class is 75% of the number of classes taught.

Learning Results

1. Know the relevant techniques in recombined DNA technology and their insertion and expression in bacteria and yeast.
2. Apply the techniques of gene manipulation in plants.
3. Know and apply genetic manipulation in animal cells.

Program

Module 1. Genetic engineering as applied science; Methods of gene manipulation; Host cells and vectors; Cloning strategies; Genetic engineering applied to plants; Understand genes, genomes and “-omas”. This module evaluates learning outcomes for objectives 1 and 2
Module 2. Genetic engineering and biotechnology; Transgenic animals. This module assesses the learning outcomes for goal 3

Curricular Unit Teachers

Grading Methods

Avaliação contínua
  • - Modulo 2: 1) teste escrito (75%); 2) participação nos trabalhos práticos (25%). - 40.0%
  • - Modulo 1: 1) teste escrito (70%); 2) relatórios dos trabalhos práticos (15%); 3) apresentação e discussão de um tema (15%) - 60.0%

Internship(s)

NAO

Bibliography

Avise, John, C. The Hope, Hype and Reality of Genetic Engineering. Oxford University Press, USA, (2004) pp 242.
Brown, T.A. Gene Cloning & DNA Analysis. An Introduction. 5th. Ed. Blackwell Publishing, UK, (2006) pp 386.
Chrispeels, M.J. and Sadava, D.E. Plants, Genes and Crop Biotechnology. 2nd ED. Jones and Bartlett Publishers, USA, (2003) pp 562.
Khachatourians, G.G., McHughen, A., Scorza, R., Nip, W. and Hui, Y.H. Transgenic Plants and Crops. Marcel Dekker, Inc., New York, USA, (2002) pp. 876.
Lima, N, & Mota, M. Biotecnologia. Fundamentos e Aplicações. LIDEL, Lisboa, Portugal, (2003) pp. 505.
Malacinski, G.M. Fundamentos de Biologia Molecular. 4ª Ed. Guanabara Koogan S.A., Brasil, (2005) pp. 439.
Neal, C. Stewart, Jr. Genetically Modified Planet. Environmental impacts of genetically engineered plants. Oxford University Press, USA, (2004) pp 240.
Nicholl, D.S. An Introduction to Genetic Engineering. 2nd Ed. Cambridge University Press, UK, (2005) pp. 292.