Base Knowledge
Técnicas laboratoriais em Farmácia, química orgânica e química inorgânica
Teaching Methodologies
Expository theoretical classes using audiovisual and interactive means between student and teacher, with the use of practical classes to reinforce the understanding of the theorist’s syllabus
Students will be monitored throughout each semester with clarification of doubts, whenever requested and as a complement to training.
Two hours a week are available, with a pre-defined schedule or to be agreed with the student.
Each student is also given the pedagogical supports of all the material taught or the
recommended bibliographical references for the discipline, at the beginning of the semester.
Learning Results
The curricular unit of analytical chemistry in pharmacy I aims to provide the student an adequate
knowledge of theoretical principles, practices and application components of instrumental
methods of analysis, essential to the performance of laboratory activities, having as main
objective the application of new technologies in the area of Pharmacy, your validation, as well as
in the area of investigation.
Also wants to enable and empower the learner on programming steps of developing an analytical
method, as well as in the selection of this, in the face of specific requirements, such as:
specificity, sensitivity, detection limit, selectivity, reproducibility and matrix interference, defining
further indicates the factors that lead to the choice of a particular method of analysis.
Competencies to develop:
-Understanding of the nature, objectives and applications of instrumental methods of analysis;
-Acquisition of knowledge and skills in the application of analytical methods in solving analytical
problems;
– Ability to define/select experiences and procedures in obtaining results;
-Ability to identify the conditions of applicability of various quantitative and qualitative analytical
methods;
-Determination of the concentration of various analytes by classical methods and instruments;
– Ability to handle and interpret analytical results
Program
Theoretical framework
1. INTRODUCTION TO ANALYTICAL CHEMISTRY – 2 hours
1.1. Qualitative analysis: chemical processes, physical, physico-chemical and thermal
1.2. Quantitative analysis: chemical processes, physical and physicochemical
1.3. Analytical process
1.4. Classical analytical methods and instruments
1.5. Comparison of analytical methods
2. ERRORS IN ANALYTICAL CHEMISTRY – 2 hours
2.1.Types of errors
2.2. Accuracy and precision
2.3. Statistical analysis of experimental data
2.4. Mean and standard deviation
2.5. Confidence interval and reliability testing
3. QUALITY CONTROL AND QUALITY SYSTEMS analytically 2hours
3.1 Quality definition
3.2 Quality requirements
3.3 Quality control system
3.4. Statistical quality control
3.5. The 7 Traditional Tools
3.6 Quality management
3.7 Quality assurance
4. ACCREDITATION AND CERTIFICATION of LABORATORIES-2h
4.1 Certification
4.2 Accreditation
5. METHODS CALIBRATION – 2 hours
5.1. Calibration curves
5.2. Standard solutions
5.3. Linear regression
5.4. Standard addition method
5.5. Internal standard
6. SAMPLING – 2 hours
6.1. Sampling plan
6.2. Sampling Techniques
6.3. Sampling Procedures
6.4. Sampling records
7. LABORATORY AUTOMATION – 2 hours
7.1. Continuous analyzers
7.2. Discontinuous analyzers
8. VALIDATION OF METHODS – 2 hours
8.1. Standardized method
8.2. Internal method
8.3. Validation by indirect evaluation
8.3.1. Practicability
8.3.2. Calibration curves
8.3.3. Working Range
8.3.4. Linearity
8.3.5. Detection limit of the method
8.3.6. Quantification limit of the method
8.3.7. Sensibility
8.3.8. Accuracy / Loyalty
8.3.9. Specificity / Selectivity
8.3.10. Robustness
8.4. Validation by direct assessment
8.4.1. Certified reference materials
9. VOLUMETRIC METHODS – 6 hours,
9.1. Types of volumetric methods and evaluation of the titration solutions
9.2. Acid-base titrations
9.2.1. Titration of acids and strong and weak bases
9.2.2. equivalence point detection methods
9.2.3. Applications with analytical interest
9.3. Oxidation-reduction titrations
9.3.1. Reactions with electron transfer
9.3.2. Methods of detection of the equivalence point in redox reactions
9.3.3. Features and reactions of some titrants used in redox volumetry
9.3.4. Applications with analytical interest
9.3. Complexing titrations
9.3.1. complexation reactions; complexing agents
9.3.2. equivalence point detection methods
9.3.3. Applications with analytical interest
9.4. Precipitation tritation
9.4.1. precipitation reactions; solubility
9.4.2. equivalence point detection methods
9.4.3. analytical applications
10. METHODS gravimetric – 2 hours
10.1. General characteristics of gravimetric methods
10.2. Precipitation
10.3. Formation of a precipitate: nucleation, crystallization and aging
10.4. Nucleation and crystal growth
10.5. Colloids
10.6. Contamination of precipitates
10.7. analytical applications
11. ELECTROCHEMICAL METHODS – 6 hours,
11.1. General principles
11.2. Types of electrochemical cells
11.3. Potential electrode (Nernst equation)
11.4. Potentiometry
11.4.1. Basic principles and instrumentation
11.4.2. Types of electrodes; indicator electrode, reference membrane and combined
11.4.3. Direct potentiometric
11.4.4. Potentiometric titrations
11.5. Voltammetry
11.5.1. Basic principles and instrumentation
11.5.2. Voltammetric cells
11.5.3. Types of electrodes; indicator electrode, reference and auxiliary
11.5.4. Selective electrodes
11.5.5. Electrodes chemically modified
11.5.6. Linear scan voltammetry
11.5.7. Cyclic voltammetry
11.5.8. Anodic stripping voltammetry
11.5.9. Cathodic stripping voltammetry
11.5.10. Adsorptive stripping voltammetry
11.6. Analytical applications
Practice matrix
1. Qualitative and quantitative analysis of compounds- 12 hours
1.1.Impurities research in the purified water
1.2. Ion identify on polyelectrolytic solution
1.3. Identification and determination of paracetamol
1.4. Quality control of potassium hydroxide
2. Volumetric Analysis – 12 hours
2.1. Oxidation-reduction tritation- Determination of a concentrated solution of hydrogen peroxide
2.2. Complexing titration- Determination of magnesium sulfate to 20% Injectable
2.3 Precipitation tritations- chlorides by Mohr method
2.4- Acid-base tritations- Standard solutions of HCL
3. Treatment graphic and statistical analysis results
4. Potentiometric
4.1. Potentiometric determination of acetylsalicylic acid
4.2. Determination of fluoride concentration using selective electrode
4.3. Determination of chloride concentration using selective electrode
Curricular Unit Teachers
Internship(s)
NAO
Bibliography
Equal