Separation and Purification Processes of Biological Products

Base Knowledge

Knowledge of Thermodynamics, Mass Transfer and Fluid Mechanics.

Teaching Methodologies

Theoretical and theoretical practices classes. The theoretical classes are intended to expose the syllabus of the course accomplished with illustrative examples of applications. The theoretical practices classes are used for solving problems and exercises. In these classes practical cases are discussed and solved in class by the students, where discussion and working in group are encouraged. The independent study is also encouraged.

Learning Results

In this course unit, the aim is for students to:

(i) identify and describe some separation techniques used in Biotechnology to be able to size the equipment used in the unit operations of solid-liquid and liquid-liquid extraction, membrane filtration, adsorption, and ion exchange;

(ii) identify and describe the equipment used in these unit operations;

(iii) write and develop the equations that allow for characterizing the operation and sizing the equipment;

(iv) demonstrate an overall understanding of the requirements for handling and validating processes involving biological products;

(v) be able to solve problems involving the studied separation processes.

Program

1. Biological processes 1.1 Biological processes of industrial interest 1.2 Main steps of the biological processes: initial processing, bioreaction and final processing 1.3 Final processing: cell removal, isolation and purification 2. Liquid- liquid and solid-liquid extraction 2.1 Extraction process in a simple step 2.2 Extraction multistep process for continuous countercurrent and crossflow 2.3 Calculation of the number of equilibrium stages in a countercurrent cascade by graphical method 2.4 Effect of solvent flow rate (minimum flow rate) 3. Membrane separation processes 3.1 Nomenclature and basic concepts 3.2 Dialysis, gas permeation, reverse osmosis, ultra-filtration, microfiltration 3.3 Applications, equipment and models for reverse osmosis 4. Adsorption and ion exchange 4.1 Adsorbents and ion exchange resins 4.2 Equilibrium adsorption and ion exchange .

Curricular Unit Teachers

Internship(s)

NAO

Bibliography

Main Bibliography

Moreira, M. J. (2024). Apontamentos de Processos de Separação e Purificação de Produtos Biológicos. ISEC (disponível na plataforma académica InforEstudante).

Pabby, A. K., Rizvi, S. S. H. & Sastre, A. M. (Eds.). (2015). Hanbook of Membrane Separations: Chemical, Pharmacerutical, Food, and Biotechnological Applications. (2ª ed). CRC Press. (COTA: 6-8-58 ISEC) 

McCabe W.L., Smith J.C., Harriote P. (2001). Unit Operations of Chemical Engineering (6ª ed.): Mc Graw-Hill. (COTA: 6-7-27 ISEC)

Azevedo, E. G. e Alves, A. M. (2017). Engenharia de Processos de Separação (3ª ed.). Lisboa: IST Press. (COTA: 6-7-200 ISEC)

Geankoplis, C. J. (2003). Transport Processes and Separation Process Principles (includes unit operations) (4ª ed.). Upper Saddle River, NJ : Prentice Hall PTR. (COTA: 6-7-70 ISEC)

Harrison, R. G.; Todd, P.; Rudge, S. R. & Petrides, D. P. (2003). Bioseparations Science and Engineering. USA: Oxford University Press. (COTA: 6-13-14 ISEC)

Supplementary Bibliography

Ahuja, S. (Ed.) (2000). Handbook of Bioseparations. San Diego. Academic Press. 

Baker, R.W. (2004). Membrane Technology and Applications. John Wiley & Sons.