Enzymatic Engineering

Base Knowledge

Not defined

Teaching Methodologies

Lecture (T) classes are expository using a PowerPoint slides projection In practical (TP) classes, after a brief theoretical introduction, the different chapters exercise sheets are solved. All study material is made available through the Moodle platform. Students are encouraged to participate with questions and comments during both lecture and practical classes.

Learning Results

The learning objectives in this course is to provide students with the ability to: i) understand the role of enzymes in biotechnological applications; ii) understand the properties and functions of enzymes; iii) identify the types of enzymatic catalysis mechanisms; iv) describe the biocatalysis process in unconventional means; v) model the enzyme kinetics; vi) understand and describe the main types of enzyme inhibition; vii) determine the effect of enzyme immobilization on their kinetics; viii) understand and model the main types of reactors with immobilized enzymes.

Program

1. Enzymes properties
Classification and nomenclature. Specificity of enzymes. Enzyme catalysis mechanisms.
2. Biocatalysis in non-conventional systems
Biocatalysis in aqueous systems, organic solvents, ionic liquids, supercritical fluids, solid-solid systems and liquid-solid systems.
3. Kinetics and enzymatic stability. Michaelis-Menten model. Alternative and complementary models. Graphical determination of kinetic constants. Enzymatic activity and stability. Effect of pH and temperature on enzyme kinetics. Types of enzyme inhibition.
4. Immobilization of enzymes
Support media types and immobilization techniques. Effects on the enzymatic properties and kinetics.
5. Reactors with immobilized enzymes
Classification. Main types of reactors with immobilized enzymes and respective kinetic models.
6. Enzyme technology
Main industrial applications of enzymes. Biosensors and enzymatic clinical diagnostics. Enzymes for industrial, pharmaceutical, food and environmental use.

Curricular Unit Teachers

Grading Methods

Avaliação por Exame
  • - Exame - 100.0%
Avaliação Contínua
  • - 2 testes realizados ao longo do semestre - 75.0%
  • - resolução individual, nas aulas teórico-práticas, de um conjunto de pequenos exercícios - 10.0%
  • - elaboração de um seminário e respetiva apresentação oral - 15.0%

Internship(s)

NAO

Bibliography

5. Engel, P. C., Enzymology Labfax, Academic Press, 1996.

2. Lima, N., Mota, M., Biotecnologia, Fundamentos e Aplicações, Lidel, Edições Técnicas, Lda, 2003.

3. Aehle, W., Enzymes in Industry – Production and Applications, Wiley, 2004.

1. Cabral, J. M. S., Aires-Barros, M. R., Engenharia Enzimática, Lidel, Edições Técnicas, Lda, 2003.

6. Fonseca, M.M., Teixeira, J.A., Reactores biológicos: fundamentos e aplicações. Lidel, 2007.

4. Bom, E.P.S., Ferrara, M.A., Corvo, M.L., Enzimas em Biotecnologia. Produção, Aplicações e Mercado. Ed. Editora Interciência, 2008.