Enzyme Technology

Chemistry and General Biochemistry

In the theoretical classes the general presentation of the subjects is carried out, being presented: the programmatic contents; examples of application of the subject matter where appropriate; topics for discussion during the class or in the followingclasses through the use of slideshow or podcasts.

In the laboratory practical classes, the fundamentals of the experimental work to be performed (at the beginning of the experimental activity), discussed and interpreted the results obtained (during and at the end of the activity) will be presented. In these classes will be provided a space for presentation and discussion of themes and their application in other contexts.

Students will be encouraged to pre-read the recommended bibliography and a critical and substantiated analysis of the results obtained in accordance with the scientific evidence learned.

Study visits may be made.

At the end of the teaching of the course unit, students should:

Identifie the theoretical and practical principles related to Enzyme Technology.

Apply knowledge and uses laboratory resources to solve problems and carry out tasks, through logical, intuitive and creative thinking, manual dexterity using materials, tools and methods.

Demonstrate ability to use knowledge, skills and personal abilities, in the context of group study, for the purposes of professional and personal development.

A. Theoretical Component

Fundamentals of Enzymatic Technology; Historical Introduction; General Properties of Enzymes; Classification and nomenclature of enzymes; Structure and function of enzymes; Kinetics of enzymatic reactions and factors that influence enzymatic activity;  Immobilization of enzymes;

Applications of Enzymes: as final product; as process additives; in food systems; in biotransformation processes.

Enzymes legal status and quality

B. Practical Component

Use of enzymes in practical laboratory work.  Determination of enzymatic activity and kinetic constants of enzyme catalyzed reactions.  Evaluation of the optimal conditions to favor the enzymatic activity and to identify experimentally the mechanisms of enzymatic inhibition

• - Teste Prático - 40.0%
• - Teste Teórico - 60.0%

NAO

CABRAL, J.M.S., AIRES-BARROS, M.R., Engenharia Enzimática, Ed. Lidel. 2007. ISBN: 972-757-272-3

LIMA, N., MOTA, M., Biotecnologia, Ed. Lidel. 2003. ISBN: 972-757-197-2

UCHHOLZ, K.,BORNSCHEUER, U.,KASCHE, V. Biocatalysts and Enzyme Technology. UK: Wiley-VCH Verlag GmbH, 2012. 626 p.