Genetic Engineering

Biology, Genetics, Molecular biology

Module 1 Gene manipulation, transformation and PCR applications Module 2 Modified organisms Theoretical and practical classes and reporting related to different themes For a better consolidation of concepts, students are accompanied weekly in tutorial classes. Students are required to attend classes. The minimum attendance rate for each type of class is 75% of the number of classes taught.

1. Know the relevant techniques in recombinant DNA technology and its insertion and expression in bacteria and yeast. 2. Apply gene manipulation techniques to plants. 3. Know and apply genetic manipulation in animal cells.

Module 1. Genetic engineering as an applied science; The methods of gene manipulation; Host cells and vectors; Cloning strategies; Genetic engineering applied to plants; Understanding genes, genomes and “-omas”. This module assesses learning outcomes for objectives 1 and 2 Module 2. Genetic engineering and biotechnology; Transgenic animals. This module assesses the learning outcomes for objective 3

• - Modulo 2: 1) teste escrito (75%); 2) participação nos trabalhos práticos (25%). - 40.0%
• - Modulo 1: 1) teste escrito (70%); 2) relatórios dos trabalhos práticos (15%); 3) apresentação e discussão de um tema (15%) - 60.0%

NAO

Avise, John, C. The Hope, Hype and Reality of Genetic Engineering. Oxford University Press, USA, (2004) pp 242. Brown, T.A. Gene Cloning & DNA Analysis. An Introduction. 5th. Ed. Blackwell Publishing, UK, (2006) pp 386. Chrispeels, M.J. and Sadava, D.E. Plants, Genes and Crop Biotechnology. 2nd ED. Jones and Bartlett Publishers, USA, (2003) pp 562. Khachatourians, G.G., McHughen, A., Scorza, R., Nip, W. and Hui, Y.H. Transgenic Plants and Crops. Marcel Dekker, Inc., New York, USA, (2002) pp. 876. Lima, N, & Mota, M. Biotecnologia. Fundamentos e Aplicações. LIDEL, Lisboa, Portugal, (2003) pp. 505. Malacinski, G.M. Fundamentos de Biologia Molecular. 4ª Ed. Guanabara Koogan S.A., Brasil, (2005) pp. 439. Neal, C. Stewart, Jr. Genetically Modified Planet. Environmental impacts of genetically engineered plants. Oxford University Press, USA, (2004) pp 240. Nicholl, D.S. An Introduction to Genetic Engineering. 2nd Ed. Cambridge University Press, UK, (2005) pp. 292.