Teaching Methodologies
Teaching and Learning Methodologies (MEA)
MEA 1. Expository methodology for the presentation of theoretical content.
MEA 2. Participatory methodology involving the analysis and resolution of practical exercises.
MEA 3. Active learning methodologies through the development of individual and group assignments.
MEA 4. Self-directed learning, associated with the student’s autonomous work.
Learning Results
Learning Objectives (LO):
LO1 – Explain the basic concepts of biotechnological processes and their importance within the scientific and industrial landscape and describe the main stages.
LO2 – Identify and describe the most relevant unit operations involved in cell removal, primary isolation, purification, and final isolation.
LO3 – Explain and apply the basic principles underlying unit operations in biotechnological processes.
LO4 – Compare mechanical and non-mechanical cell disruption methods, indicating their advantages, limitations, and suitability for different types of biomass.
LO5 – Describe the operation of the most commonly used equipment for cell disruption.
LO6 – Perform solid-liquid extraction procedures using plant matrices.
LO7 – Quantify the content of bioactive compounds (phenolics) and the antioxidant activity of plant extracts.
LO8 – Analyze and critically discuss the experimental results and prepare a technical-scientific written report.
Program
Module I
MI 1. Introduction. Characterization of biotechnological processes. Main stages. Importance of biotechnological processes in the global context.
MI 2. Downstream processing: cell removal, primary isolation, purification, and final isolation. Main unit operations used in each stage.
MI 3. Separation of insoluble products: filtration and centrifugation. Available equipment. Theoretical concepts and calculation of operating conditions.
Module II
MII 1. Cell disruption: mechanical and non-mechanical methods. Key features of the most common equipment.
MII 2. Separation of soluble products: extraction and adsorption. Extraction columns – concept of ideal stage. Adsorption isotherms. Available equipment. Determination of operating conditions.
Module III
MIII 1. Laboratory practice: conventional solid-liquid extraction of plant matrices. Quantification of phenolic compounds in the extracts. Discussion of results and preparation of the written report.
Curricular Unit Teachers
Maria João Mendes Cardoso Barroca DiasInternship(s)
NAO
Bibliography
DORAN, Pauline (2012). Bioprocess Engineering Principles, 2nd Edition. Academic Press, London. ISBN: 978-0122208515.
FLICKINGER, Michael C. (2013). Downstream Industrial Biotechnology: Recovery and Purification. John Wiley & Sons, New Jersey. ISBN: 978-1-118-13124-4
FORCINITI, Daniel (2008). Industrial Bioseparations: Principles and Practice. Wiley-Blackwell, Oxford. ISBN: 978-0-8138-2085-91.
GEANKOPOLIS, C. J.; HERSEL, A., A.; LEPEK, D., H. (2018). Transport Processes and Separation Process Principles (5th ed.) Prentice Hall, Boston. ISBN-13: 978-0-13-418102-8.
HARRISON, Roger G.; TODD, Paul; RUDGE, Scott R.; PETRIDES, Demetri P. (2015). Bioseparation Science and Engineering, 2nd Edition. Oxford University Press, Oxford. ISBN: 978-0-19-539181- 7.
SHULER, Michael; KARGI, Fickret; DELISA, Mathew (2017). Bioprocess Engineering: Basic Concepts, 3rd Edition. Prentice Hall, Boston. ISBN13: 978-0137062706.