Microbiology Laboratory

In the laboratory classes of microbiology, 8 experimental works are carried out, and the protocols will be made available to the students, previously, through the Moodle platform. Students work in a group of 2 or 3 elements. After the experimental work is done, the students will have a week to prepare a summary report, which will be given to the teacher and discussed with them. In some laboratory classes students will perform, at the beginning of the class, a surprise test for diagnostic evaluation of the experimental protocol that will be executed.

The main objective of the Training Unit is to introduce the student to the laboratory techniques of Microbiology, with a special emphasis on basic procedures such as the preparation of culture media, sterilization and asepsis techniques, inoculation techniques, staining techniques of bacterial cell components and biochemical characterization of bacteria.
Students at the end of the course unit should know:
– Preparing culture media
– Use sterilization and asepsis techniques
– Recognize microorganisms in the environment: colony morphology
-Transfer and isolate microorganisms under aseptic conditions
– Conserving and reviving cultures
– Apply the staining techniques of bacterial cell components
– Characterize physiologically and biochemically bacteria
– Evaluate the effectiveness of antibiotics, antiseptics and disinfectants
– To recognize the methods of quantification of microorganisms
– Determine a microbial growth curve.

– Sterilization of Laboratory material.
– Preparation of culture media, isolation and inoculation techniques.
– Micro-organisms in the environment.
– Staining techniques: staining with methylene blue and Gram staining.
– Biochemical and metabolic characterization of bacteria.
– Methods for quantification of microbial growth.
– Curve and bacterial growth rate.

NAO