Base Knowledge
Not applicable.
Teaching Methodologies
In the laboratory classes of microbiology, 5 experimental works are carried out, with the protocols previously made available to the students, through the NONIO platform. Students work in a group of 2 or 3 elements. After the experimental work is done, the students will have a week to prepare a summary report, which will be given to the teacher and discussed with them. In some laboratory classes students will perform, at the beginning of the class, a surprise test for diagnostic evaluation of the experimental protocol to be executed.
Learning Results
The main objective of the course is to provide students with practice in microbiology laboratories.
It is intended that students acquire the following knowledge and skills: i) Prepare culture media and use sterilization and aseptic techniques. Ii) Transfer and isolate cultures under aseptic conditions. iii) Physiologically and biochemically characterize microorganisms. iv) Experimentally quantify the number and biomass of microorganisms and their growth. v) Evaluate the effectiveness of antimicrobial agents; vi) Microbiologically analyze water and food products.
Program
Sterilization and asepsis techniques. Preparation of culture media. Transfer and isolation of cultures under aseptic conditions. Physiological and biochemical characterization of microorganisms. Growth and quantification of microorganisms. Assessment of the effectiveness of antimicrobial agents. Microbiological analysis of water and food products.
Curricular Unit Teachers
António Luis Pereira AmaralGrading Methods
Continuous evaluation. Frequency of laboratory classes is required.
The evaluation of the Microbiology Laboratory course unit will be continuous throughout the semester and will take into account:
(C1) Evaluation of Experimental Work: Elaboration and evaluation of 5 summarized reports related to the Experimental Works, carried out in groups, discussion and defense of the Experimental Works carried out (40% of the final grade).
(C2) Evaluation of the Experimental Work: Performance evaluation during laboratory classes. Students will be evaluated in all classes of the curricular unit by the interest shown, level of learning, work dynamics of each student in group, punctuality and attendance (15% of the final grade).
(C3) Written test: constitutes an element of individual assessment (30% of the final grade), carried out in the last class.
(C4) Mini-tests: completion of a total of 3 to 4 surprise mini-tests, to be carried out at the beginning of some classes (15% of the final grade).
The final classification (CF) is obtained by summing the contributions of the various components: CF=0.4*C1+0.15*C2+0.3*C3+0.15*C4
In order to pass the Microbiology Laboratory course unit, the student must have a final grade (CF) of 10 values and cannot have a grade lower than 7.5 (from 0 to 20) in C3 component.
The applicable attendance regime is defined in the Coimbra Polytechnic Institute Regulations for the Professional Technical Higher Courses.
Internship(s)
NAO
Bibliography
- Amaral, A. L. (2015) Laboratório de Microbiologia: Protocolos experimentais (available at InforEstudante)
- Black, G. (2005). Microbiology – principles and explorations (6ª ed.). NJ: John Wiley & Sons (available at ISEC library: 6-14-11). ISBN: 978-0471420842
- Willey, J. M., Sherwood, L. M., Woolverton, C. J. (2008). Microbiology (7ª ed.). Boston: McGrawHill Higher Education (available at ISEC library: 6-14-8). ISBN: 978-0072992915
- Isaac, S. & Jennings, D. (1995). Microbial Culture, Bios Scientific Publishers (available at ISEC library: 6-14-10). ISBN: 978-1872748924
- Collins, C. H., Lyne, P. M., Grange, J. M. (1995). Microbiological Methods (7ª ed.). Oxford: Hodder Education Publishers. ISBN: 978-0750606530
- Seeley, H. W., Vandermark, P. J., Lee, J. J. (1991). Microbes in action: a laboratory manual of microbiology (4ª ed.). New York: W. H. Freeman and Company (available at ISEC library: 6-14-5). ISBN: 978-0716721000
- Cappuccino, J. G., Sherman, N. (1999). Microbiology: a laboratory manual (5ª ed.). Menlo Park, CA: Benjamin/Cummings Science Publishing. ISBN: 978-0805376463