Base Knowledge
Not applicable
Teaching Methodologies
Lecture classes will focus on the curricular unit core notions whereas practical classes will focus on exercise sheets resolution by the students. Lecture classes will take advantage of powerpoint presentations projection and in the practical classes the exercise sheets correct answers will be revealed at the end of the class. The study material is available through the platform adopted by ISEC.
Learning Results
This course aims to explore various instrumental techniques in chemical and biological analysis. The objectives are for students to acquire the following knowledge and skills: i) Recognize the applications of instrumental techniques in chemical analysis; ii) Understand the instrumentation and the physicochemical principles of each analytical method; iii) Identify the practical aspects of instrumental methods; iv) Recognize the main rapid methods for the detection and quantification of microorganisms; v) Recognize the main molecular biology and immunological methods for the detection of microorganisms and toxins; vi) Identify microscopy techniques for the identification of microorganisms and ultrastructure.
Program
1. Complementary Methods of Chemical Analysis:
Qualitative and quantitative analyses. Optical methods (atomic and molecular absorption – UV/VIS; flame photometry; atomic emission, FTIR, Raman, ICP; fluorescence, phosphorescence). Mass spectrometry. Electroanalytical methods (potentiometric, voltametric, coulometric, and electrogravimetric methods).
2. Complementary Methods of Biological Analysis:
Rapid methods for the determination and quantification of microorganisms. Molecular biology methods for the identification of microorganisms. Immunological methods for the identification of microorganisms and toxins. Microscopy techniques for the identification of microorganisms and ultrastructure.
Curricular Unit Teachers
António Luis Pereira AmaralInternship(s)
NAO
Bibliography
1. Levine, I.N. (1995). Physical Chemistry. McGraw Hill, NY. Disponível na biblioteca: 6-5-40 (ISEC) – 08685
2. Rouessac, F. & Rouessac, A. (2000). Chemical analysis : modern instrumental methods and techniques. John Wiley. Disponível na biblioteca: 6-4-3 (ISEC) – 11339
3. Skoog, D.A., Leary, J.J. (1992). Principles of Instrumental Analysis. 4th Edition, Saunders College Publishing. Disponível na biblioteca: 6-4-33 (ISEC) – 06151
4. Harris, D.C. (2001). Quantitative Chemical Analysis. W.H. Freeman and Company, New York, 5th Edition. Disponível na biblioteca: 6-3-72 (ISEC) – 11880 ; 6-3-72CD (ISEC) V.CD-ROM – 11880CD
5. Gonçalves, M.L.S.S. (2001). Métodos Instrumentais para a Análise de Soluções: Análise Quantitativa, Fundação Calouste Gulbenkian, Lisboa, 4ª Edição. Disponível na biblioteca: 6-4-46 (ISEC) – 13096 ; 6-4-43 (ISEC) – 13987. – Ex. repetido
6. Azevedo, C. (2005). Biologia Celular e Molecular. Lisboa: Lidel. (disponível na biblioteca do ISEC: 6-15-19)
7. Robertis, E., Robertis Jr., E. M. (2005). Biologia Celular e Molecular. Lisboa: Fundação Calouste Gulbenkian. (disponível na biblioteca do ISEC: 6-15-15)
8. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2002). Molecular Biology of the Cell. New York: Garland Science. (disponível na biblioteca do ISEC: 6-15-20).
9. Pelczar, M.J., Chang, E.C.S., Krieg, N.R. (1996). Microbiologia: conceitos e aplicações. São Paulo: Makron Books. (disponível na biblioteca do ISEC: 6-14-13 – 14170)
10. Willey, Sherwood, Woolverton (2008). Prescott, Harley and Klein’s Microbiology. Boston: McGraw Hill. (disponível na biblioteca do ISEC: 6-14-8 – 13997).
11. Black. G. (2005). Microbiology: principles and explorations. New Jersey: John Wiley & Sons. disponível na biblioteca do ISEC: 6-14-11 – 14165).