Métodos Complementares de Análise Química e Biológica

Not applicable  

Lecture classes will focus on the curricular unit core notions whereas practical classes will focus on exercise sheets resolution by the students. Lecture classes will take advantage of powerpoint presentations projection and in the practical classes the exercise sheets correct answers will be revealed at the end of the class. The study material is available through the platform adopted by ISEC.

This course aims to explore various instrumental techniques in chemical and biological analysis. The objectives are for students to acquire the following knowledge and skills: i) Recognize the applications of instrumental techniques in chemical analysis; ii) Understand the instrumentation and the physicochemical principles of each analytical method; iii) Identify the practical aspects of instrumental methods; iv) Recognize the main rapid methods for the detection and quantification of microorganisms; v) Recognize the main molecular biology and immunological methods for the detection of microorganisms and toxins; vi) Identify microscopy techniques for the identification of microorganisms and ultrastructure.

1. Complementary Methods of Chemical Analysis:

Qualitative and quantitative analyses. Optical methods (atomic and molecular absorption – UV/VIS; flame photometry; atomic emission, FTIR, Raman, ICP; fluorescence, phosphorescence). Mass spectrometry. Electroanalytical methods (potentiometric, voltametric, coulometric, and electrogravimetric methods).

2. Complementary Methods of Biological Analysis:

Rapid methods for the determination and quantification of microorganisms. Molecular biology methods for the identification of microorganisms. Immunological methods for the identification of microorganisms and toxins. Microscopy techniques for the identification of microorganisms and ultrastructure.

The students can choose one of the following evaluation methodologies: (i) periodic evaluation by a total of 2 interim evaluations; (ii) assessment by final written exam quoted to 20 values (100%). Students who complete all theoretical periodic assessment instruments do not have access to the “normal” period exam. In the written tests it is not allow the consultation of any document or use of any electronic equipment.

The Final Classification of the curricular unit will result from the following equation: CFinal = 0.50×T1 + 0.50×T2, where T1 is the classification of the 1st test and T2 the classification of the 2nd test. To obtain approval for this course students must obtain a final grade equal to or greater than 9.5 values (from 0 to 20).

The applicable attendance regime is defined in the Coimbra Polytechnic Institute Regulations for the Professional Technical Higher Courses: “Whenever a student is eligible for funding for a CTeSP, namely, from the Structural Funds of the European Union, it is mandatory to attend at least 75% of the total class hours of each course unit in order for the student to be assessed under continuous/periodic evaluation, and at least 50% for the student to be assessed through examination, without prejudice to the situations provided for by law.”  

NAO