Technological Laboratory

Transmission of theoretical knowledge followed by practical demonstration to be carried out by the students, for each topic covered.

This course unit, framed within the present degree, aims to address relevant techniques in the field of Biotechnology with practical and/or laboratory application not covered in other course units, whenever possible involving experimental demonstration and from the point of view of technique construction. The topics to be covered may vary throughout the development and evolution of the course.

1) Research of technical-scientific information. Use of databases. Use of VPN networks. Patents. 2) Phosphorescence, fluorescence and bioluminescence. Applications: Dynamic phototherapy: others. 3) Capillary electrophoresis. Preparation of capillaries for GC and EC. Contributions to the advancement of biotechnology: characterization of microorganism populations; recent developments. 4) Mass spectrometry (MS) and its potential. MALDI-MS. Structural identification by GC-LC-MS. Applications in metabolomics. Application: determination of the profile of compounds in biological samples. Solid-phase extraction techniques: use of SPE and SPME.

1) Vertical gel electrophoresis (SDS-PAGE). Applications: determination of the protein profile in samples. 2) Bioengineering: Control and automation techniques: Analog and digital signals. Analog-to-digital conversion. Sensors. Basic notions of control: proportional control, PID. Labview. Practical implementation of a remotely controlled reactor.

NAO