Culture of Animal Cells

The teaching and learning process is based on the following teaching methodologies:

a) Lectures of theoretical / practical classes;

b) Execution of practical works of animal cell culture in the laboratory and in the classroom by simulation;

c) Bibliographic research, article analysis and presentation of papers.

Practical laboratory classes on cell growth and maintenance in culture enable students to integrate their knowledge, promoting autonomy, critical thinking and work skills.

The program organized into two modules and periodic assessment is used to encourage continuous study and facilitate the acquisition of skills throughout the semester.

The students during their lectures and presentations are encouraged to seek the latest investigations being conducted in diverse fields of animal and human health to promote their interest in the field of investigation and in future seek to join the different research units available in the country and abroad.

Animal cell culture is an important technique in many molecular biology, development biology and biotechnology laboratories. It is a technique that is becoming increasingly important in research in the field of biology, tissue replacement therapy, therapeutic proteins and others. This technique allows animal tissue models to be constructed and used as alternatives to experimentation on live animals.

The learning objectives of this UC are intended to provide the student who has successfully completed the following competencies:

1) To understand animal cell culture techniques and the functioning standards of a cell culture laboratory.

2) To know the constituents of the culture medium and strategies of formulation of culture media for animal cells.

3) To understand the aseptic techniques of manipulation and maintenance in the culture of animal cells

4) To know the applications of cell culture techniques in animal health and breeding.

Module 1:

History of animal cell culture (ACC). Advantages and limitations. Types of tissues. Applications of the ACC technique.

Ideal environment for ACC: conditions and equipment; ACC laboratory design.

Media used in ACC.

Classification and characteristics of ACC: primary and secondary cell culture. Organotypic and histotypic cultures.

Growth and maintenance of cells in culture. Cell line and culture monitoring.

Contamination of cultures.

Module 2:

Methods for characterizing cell lines.

Cell differentiation: transformation and immortalization.

Cryopreservation of cells.

Stem cells: characterization, processing, and reprogramming.

Specialized cell culture: protocols for culturing epithelial, mesenchymal, neuronal, hematopoietic, and gonadal cells.

ACC in research: studies on nutrition, toxicology, therapeutic effects, vaccine and growth hormone production, and studies on transgenic animals.

Tissue and organ engineering.

NAO