Teaching Methodologies
Theoretical classes aim at a detailed presentation of the program using an expository and interactive methodology, appealing to the students’ intervention.
In practical classes, practical work will be carried out following the protocols, with subsequent interpretation and discussion of the results.
Continuous assessment will result from adding the theoretical matrix (70%) with practice (30%). The theoretical will comprise an individual written test (60%) and presentation of a group work or scientific articles (10%). It will involve theoretical material and works. The practice will be individual with practical exam, report and written test (30%). Each test must have at least 9 (nine) values. The approval results from the weighted average of the tests, with a score equal to or higher than 10 values.
The final exam will take place at the times covered in the academic calendar and according to the terms of the academic regulation.
Learning Results
Understand, at the molecular levei, the cellular processes and the importance of cytohistological functioning and regulation, with a view to supporting clinicai diagnosis and monitoring of pathologies;
Conventional and automated methodologies for nucleic acid extraction and amplification and subsequent qualitative and quantitative evaluation.
Recognize and apply concepts and principies of molecular biology;
Select the most appropriate methodologies for the biological product, analysis and pathology;
Analyze, synthesize, process and communicate information using appropriate methodologies;
Develop an attitude of criticai analysis based on the work done.
Present the ability to apply new methodologies of molecular biology, in response to clinicai and laboratory diagnosis and monitoring of pathologies in the areas of intervention of biomedical laboratory sciences.
Program
Molecular diagnosis in health and biomedicine; Function of nucleic acids: DNA, DNAmit, RNA, miRNAs; Epigenetics; Bioinformatics as a tool in the molecular biology laboratory; Extraction, quantification and evaluation methodologies for nucleic acids in biological products (conventional and automated methods); Polymerization chain reaction (PCR): varieties and laboratory application; Sanger sequencing and application; NGS (next generation sequencing); Massive paraliel sequencing.
Practice
Collection of biological samples to perform laboratory techniques; Application of methodologies to biological samples,
extraction and analysis of nucleic acids and subsequent interpretation of results: Extraction (DNA, RNA, microRNAs); Quantification and evaluation of the degree of purity of nucleic acids; Agarose gel electrophoresis; Polymerization chain reaction (PCR and qPCR); Restriction enzymes (RFLP); cDNA synthesis; Cloning
Internship(s)
NAO
Bibliography
– Lodish H, Berk A, Zipursky S L, Matsudaira P, Baltimore D, Damen J. Molecular Cell Biology. 5th Ed. 2004. W H Freeman and company. New York.
– Lodish H, Berk A, Matsudaira P, Kaiser C, Krieger M, Scott M, Zipursky S L, Damen J. Biologia Celular e Molecular. 5a ed. 2007. Artmed. São Paulo. Brasil.
– Strachan T, Read A P. Genética Molecular Humana. r ed. 2002. Artmed. São Paulo. Brasil.
– Azevedo C e Sunkel C E. Biologia Celular e Molecular. 5a ed. 2013. LIDEL. Lisboa.
Bibliografia secundária:
– Clark D, Pazdernik N, McGehee M. Molecular Biology. 3a ed. 2019. Academic Press. United Kingdom.