Teaching Methodologies
In the theoretical classes, there will be a detailed presentation of the program contents, using an expository, interrogative and active methodology with direct student’s participation.
In the practical classes will be use a demonstrative methodology of the concepts learned, with resolution and discussion of practical problems, Implementation of individual techniques and microscopic observation of histological slides.
Theoretical assessment:
Two Individual written tests (70%), with the minimum of 10 values in each.
Practical assessment:
Practical Assessment (20%) and group work (10%), with the minimum required of 10 values in each component. Final exam:
There is also the possibility of conducting final exam.
Learning Results
The student must acquire knowledge:
1. Physico-chemical principies of histochemistry.
2. Dye classification and stain classification.
3. Identification of histochemical stains for different tissue components.
4. Basic principies of immunocytochemistry.
5. Analytical and pre-analytical factors that affect immunostaining.
6. Immunomarking methods.
7. Quality control in immunohistochemistry.
The student must acquire skills:
1. Program and perform the Histochemical and lmmunohistochemical methods complementary to the diagnosis.
2. Select and prepare solutions, stainings and dilutions of antibodies.
The student must acquire skills:
1. Apply and control histochemical and immunohistochemical methodologies in a biomedical context, taking into account the cellular or tissue target.
2. Plan and control the histochemical staining and immunostaining procedures
3. Know the application of histochemistry and immunohistochemistry in the diagnosis / prognosis of pathologies and in targeted therapies
Program
Theoretical:
– General concepts of Histochemistry; – Types of dyes and stainings;
– Characterization of staining techniques for connective tissue, nervous tissue, carbohydrates, lipids, amyloid,
microorganisms and pigments and minerais; – General concepts of lmmunocytochemistry;
– Principie and methods for antigenic recovery; – Immunostaining methods;
– Controls in immunocytochemistry;
– Main antibody paneis: colorectal, breast, lung and lymphoproliferative tumors.
Practical:
– Perform histochemical techniques studied in theoretical classes. – Perform and optimize immunohistochemistry protocols
Curricular Unit Teachers
Diana Raquel Fernandes MartinsInternship(s)
NAO
Bibliography
1. Bancroft, J. & Gamble, M. (2002). Theory and Practice of Histological Techniques (5.aed.). London: Churchill Livingstone.
2. Moral, Raimundo Garcia dei (1993). Laboratório de Anatomia Patológica ela ed.). Madrid: McGraw-Hil I – Interamericana de Espana.
3. Carson, Freida (2000). Histotechnology: A Self-Instrumentation Text (r Ed.). American Society Clinicai Pathology
4. Elias, J. Immunohistopathology: a practical approach to diagnosis (2a Ed). Nova Iorque: American Society for Clinica! Pathology, 2003.
4. Hayat, M. Microscopy, lmmunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy. Nova Iorque: Kluwer Academic/Plenum Publishers, 2002.
5. Polak, J; Noorden, S. lntroduction to lmmunocytochemistry (3a Ed.). Londres: BIOS Scientific Publishers, 2003.