Microbiology

Biology and Biochemistry

The theoretical contents of the curricular unit will be exposed by audiovisual means, interactive classes. In the theoretical classes, the students being involved in reflection to apply the acquired Knowledge through activities such as the analysis and discussion of cases of the specialty.  In the P matrix will be developed practical laboratory work dedicated to research, enumeration and identification of microorganisms, application of disinfection and sterilization techniques and development of technical skills in the study of compounds with antimicrobial activity

The curricular unit of Microbiology aims to learn: the basic principles of medical microbiology, basic concepts of the immune response and general principles of laboratory diagnosis. In terms of bacteriology, is impotant acquired knowledge about the mechanisms of bacterial pathogenesis, its laboratory diagnosis and use the species of the greatest clinical interest. Is also important to know the antibacterial agents used in treatment (action and efficacy). In terms of virology, mechanisms of viral pathogenesis and antiviral agents. In Mycology the pathogenesis of fungal diseases and antifungal agents. In parasitology the pathogenesis of parasitic diseases and antiparasitic agents.

At the end of the course unit students should be able to: know the classification, structure and replication of bacteria, fungi, viruses and parasites; Means of control and study of microbial growth; General characteristics in laboratory diagnosis; Recognize mechanisms of pathogenesis; Recognize antibacterial, antiviral, antifungal and antiparasitic agents. To identify methods of analysis for the study and development of new compounds for the treatment of microbial infections

THEORETICAL PROGRAM (30H)

A – INTRODUCTION (1 H)

– Presentation of the contents, objectives, competences to be acquired, evaluation methodologies and recommended bibliography

– Book the evaluation periods

B – Principles of Medical Microbiology (6H)

– Classification, structure and bacterial replication

– Metabolism and bacterial genetics

– Classification, structure and replication of viruses, fungi and parasites

– Microbiota commensal and pathogenesis in humans

C- Basic Concepts of Immune Response to Microorganisms (2H)

D – BACTERIOLOGY (10H)

– Mechanisms of bacterial pathogenesis

– Staphylococcus and related Gram positive cocci

– Streptococcus

– Enterococcus and Other Gram-positive Cocci

– Enterobacteriacceae

– Pseudomonas and related microorganisms

– Gram-positive and Gram-negative Anaerobic bacteria

– Antibacterial agents and resistance phenomena

E – MICOLOGY (4H)

– Pathogenesis of fungal diseases

– Mycoses and other fungal infections

– Mycotoxins and Mycotoxicoses

– Antifungal agents

F – VIROLOGY (2H)

– Mechanisms of viral pathogenesis

– Antiviral Agents

G – PARASITOLOGY (3H)

 – Pathogenesis of parasitic diseases

-Antiparasitic agents

H- FREQUENCY (2H)

PRACTICAL PROGRAM (30H)

1 – The Microbiology Laboratory: Biosafety standards; (2H)

      Control of bacterial growth: sterilization, disinfection and antisepsis

2 – Microscopy principles and application; (4H)

      Fresh examination and bacterial stains (Gram)

3- In vitro culture, principles and applications (16H).

Rapid bacterial genus/family identification tests Identification of bacterial species;molecular and serological diagnosis.

Methods for determining the antibacterial activity of various products (natural, chemical, etc…) -well and disk diffusion tests; microdilution tests determining MIC and cytotoxicity

4- Laboratory diagnosis of viral, fungal and parasitic diseases (6H)

5 – Practical frequency (2H)

The assessment of the curricular unit is continuous and comprises two components: Theoretical, accounting for 70% of the final grade, and Laboratory Practice, accounting for 30%. The Theoretical component will be assessed through a written test covering the theoretical knowledge taught. The Laboratory Practice component will be assessed through a final practical laboratory exercise, randomly selected from the topics and methodologies covered in the practical classes, allowing the application of the knowledge and laboratory skills acquired.Students must obtain a minimum grade of 9.5/20 in each component. To pass the curricular unit, students must obtain a final grade of at least 10/20, calculated as the weighted average of the grades obtained in the two components.During the scheduled examination periods, students may be assessed through a written examination and a practical examination, structurally equivalent to the respective components of the continuous assessment.

NAO