Teaching Methodologies
Theoretical classes will include content presentations based on the syllabus of the discipline. Multimedia presentations will be used to enhance learning, encouraging direct student participation through the description and discussion of physiological mechanisms.
In the theoretical-practical aspect of physiology teaching, emphasis will be placed on video visualization to illustrate specific syllabus topics, as well as research, presentation, and discussion of scientific articles on recent advancements related to the course content.
The assessment of the course unit will involve a written evaluation with questions on the content covered in the classes.
Approval Criteria
• The final grade will be determined based on the weighting of the above components.
• To pass, students must achieve a minimum score of 10 (on a scale of 0 to 20) in the weighted average of continuous assessment and the final project.
• Attendance and active participation are encouraged, with a mandatory presence of at least 75% of the sessions.
Learning Results
1) Understand the fundamentals of the main methods used in omics and their respective instrumental analysis bases;
2) Recognise the relevance of omics, the study of the microbiome, and methods for evaluating energy metabolism, as well as the role of biomedical imaging methods in characterising metabolism in clinical and research contexts.
3) Develop autonomy in advanced laboratory protocols, critically analyse results, and apply knowledge in scientific research and clinical practice, demonstrating scientific rigour and problem-solving skills.
Program
1. Laboratory Methods for Metabolic Assessment
1.1. Electrophoretic and Chromatographic Separation Methods: Electrophoretic techniques; Chromatographic techniques
1.2. Spectroscopic Methods: Mass Spectrometry (MS); Nuclear Magnetic Resonance (NMR) Spectroscopy
1.3. Hyphenated Techniques: LC-MS; GC-MS; CE-MS
2. Methods for Energy Metabolism Assessment
2.1. Indirect Calorimetry
2.2. Respiratory Gas Analysis and Cellular/Mitochondrial Metabolism
3. Biomedical Imaging Methods
4. Genomics, Proteomics, Glycomics, Lipidomics, and Metabolomics
4.1. Concepts, Tools, and Integration of Multi-Omics Approaches in Metabolic Studies
4.2. DNA Sequencing Techniques & Gene Expression Analysis (qPCR; RNA-Seq)
4.3. Protein Extraction, Quantification, and Proteomics:
• Mass Spectrometry-Based Proteomics (LC-MS/MS)
4.4. Targeted and Untargeted Metabolomics Analysis: NMR; GC-MS
5. Microbiome Assessment: Next-Generation Sequencing (NGS) and Microbiota Analysis
Internship(s)
NAO
Bibliography
Skoog, D., Holler, F., & Crouch, S. (2017). Principles of Instrumental Analysis (7th ed.). Cengage Learning Gross, M. L. (2011). Highesolution
Mass Spectrometry: A Practical Guide. Springer.
Wilson, I. D., & Nicholson, J. K. (2017). Hyphenated Techniques in Metabolomics. Springer.
Kuster, B., & Gernot, Z. (2013). Proteomics and Genomics in the Study of Metabolism. Springer.
Varki, A., Cummings, R. D., Esko, J. D., Freeze, H. H., Stanley, P., Bertozzi, C. R., Hart, G. W., & Etzler, M. E. (Eds.). (2017). Essentials of Glycobiology (4th ed.). Cold Spring Harbor Laboratory Press.
Huang, Y., & Zhang, Y. (2016). Lipidomics: Methods and Applications. Springer.
Chen, F., & Zhang, L. (2015). Multi-Omics Data Integration in Bioinformatics. Springer.
Ranjan, R., et al. (2016). Next-Generation Sequencing: Advances, Applications, and Challenges. Wiley.