Technological Laboratory

The course first year complete.

Theoretical classes – Elucidation of the principles of operation of the different techniques and presentation of examples of application.
Practical classes – Execution of previously established protocols; bibliographical research and reports on work realized in class.

The curricular unit, within the scope of this degree, aims to approach relevant techniques in the area of Biotechnology with practical and / or laboratory use not addressed in other UC’s of the course, whenever possible involving the experimental demonstration and from the point of view of construction of the techniques. The topics to be addressed may vary during the development and evolution of the course, depending on the competencies and equipment then acquired by the School, as well as their relevance and updating.

Module 1
1) Information search. Use of databases. Use of VPN networks. Conducting research work on scientific articles.
2) Phosphorescence, fluorescence, and luminance.
Applications: Dynamic phototherapy: a new tool in the selective killing of cells.
Use in flow cytometry detectors.
3) Capillary electrophoresis. Preparation of capillaries for GC and EC. Contributions to the advancement of biotechnology: characterization of populations of microorganisms; recent developments.
4) Mass spectroscopy (MS) and its potentialities. MALDI-MS. Structural identification by GC-LC-MS. Applications in metabolomics.
Application: determination of the profile of compounds in biological samples. Solid phase extraction techniques: use of SPE and SPME.
Module 2
1) Vertical gel electrophoresis (SDS-PAGE). Applications: determination of the protein profile in samples.
2) Control and automation techniques: Analog and digital signals. Digital analog conversion. Sensors. Basics of control: proportional control

• - Modulo 2 - 50.0%
• - Modulo 1 - 50.0%

• - modulo 1 -teste escrito - 50.0%
• - molulo 2- teste escrito - 50.0%

NAO