Laboratory Methods and Techniques

•Theoretical fundaments underlying the instrumental methods discussed, as well as the function of the main
components of the instruments used, its limitations and potentialities;
•Biomedical applications underlying the electrochemical methods, immunological, spectroscopic, chromatographic
and electrophoretic;
•Understand the fundamental concepts of analytical methods studied in order to be able to draw analytical
qualitative and/or quantitative conclusions;
•Handle in a scientifically correct, expeditious and safe way the laboratory instrumentation;
•Select the most appropriate methodology to solve a problem of laboratory analysis, taking into account specific
requirements as detection sensitivity, specificity and reproducibility among others;
•Interpret critically the results and assess their quality;
•Develop capacities at the level of teamwork;
•Practical use of instrumental methods of analysis more common in clinical laboratory;

-Introduction to laboratory methods and techniques: understanding of analytical accuracy, calibration curves.
-Immunological Methods: theoretical foundations, generic applications and biomedical applications; types of
immunological tests.
-Electro analytical Methods: theoretical foundations, generic applications and biomedical applications;
potentiometry, conductimetry; coulometry.
-Spectroscopic Methods: theoretical foundations, generic applications and biomedical applications; molecular
absorption spectrometry; atomic absorption spectrometry; flame emission spectrometry; nephelometry;
turbidimetry; mass spectrometry; nuclear magnetic resonance.
-Chromatographic and electrophoretic separation methods: General principles, generic applications and
biomedical applications; SDS-PAGE electrophoresis; two-dimensional electrophoresis; capillary electrophoresis;
western blotting; thin layer chromatography; column chromatography; hyphenated techniques.

NAO