Biochemistry II

The theoretical contents of the curricular unit will be presented through lectures, illustrated whenever possible with practical case-studies and exercises in which students are encouraged to apply the competences acquired.
The teaching methodologies will also include group work dedicated to advanced search, critical review and discussion of relevant scientifc papers.
The assessment methodologies have for basis the Academic Regulation of ESTESC
PERIODIC ASSESSMENT (minimum grade: 9 out of a total of 20, per moment of assessment)
(65%): 1 written test
(15%): Bibliographic advanced search, oral presentation, discussion of a case study
(20%): Assessment of the practical syllabus
ASSESSMENT BY EXAMINATION: 80% (1 written theoretical test) + 20% (assessment of lab work)

1- Understand the main metabolic routes, its regulation/integration
2- Understand the metabolic specificities of the different tissues
3- Recognize the metabolic mechanisms of adaptation in fasting, exercise, diet or disease
4- Be familiar with biomarkers for the diagnosis/monitoring of treatment of inborn errors of metabolism
5- Be familiar with case studies of inborn errors of metabolism and its dietetic treatment
6- Practical laboratory programme: Be familiar with the experimental techniques for the quantification of analytes of clinical interest
At the end of the curricular unit, students should be able to:
– Evidence of an integrated view of the metabolism and its regulation, in physiological conditions or in adaptation
– Identify specific biomarkers for the diagnosis of inborn errors of metabolism or for the monitoring of treatment
Practical laboratorial programme: Execute experimental techniques adequate to analyse the analyte of interest

1. BIOENERGETICS
2. METABOLISM and REGULATION
2.1 Metabolism of carbohydrates
– Glycolysis; Lactic fermentation; PDH; Glycogenesis; Glycogenolysis; Gluconeogenesis
– Inborn errors of carbohydrate metabolism: Case studies and biomarkers
2.2 Lipid metabolism
– Fatty acids catabolism
– Inborn errors of lipid metabolism: Case studies and biomarkers
2.3 Metabolism of amino acids
– Amino acids catabolism; urea cycle
– Inborn errors of amino acid metabolism: Case studies and biomarkers
2.4 Integration of metabolism – Citric acid cycle; Oxidative phosphorylation
– Metabolism adaptation in fasting, exercise, diet and disease
3. Practical Laboratory Programme
– Methods and techniques of cell fractionation
– Study of metabolic pathways

NAO