Teaching Methodologies
The theoretical contents of the curricular unit will be presented through lectures, illustrated whenever possible with practical case-studies (PBL-Problem-based learning) in which students are encouraged to apply the competences acquired.
The teaching methodologies will also include (TP framework) work for the development of advanced research, critical review, and discussion of relevant scientific papers in the field of expertise and case studies.
The assessment methodologies have for basis the Academic Regulation of ESTESC.
PERIODIC ASSESSMENT
Theoretical (T) contents (60%): 1 written test (minimum grade: 9 out of a total of 20)
Theoretical-practical (TP) (40%): Oral presentation and discussion of a work based in a scientific paper in the field of expertise or case study.
ASSESSMENT BY EXAMINATION (T+TP-100%)
Learning Results
1 – Holistic understanding of the cell’s functioning, and identification of the organelles, and the relation structure/function;
2- Importance of the interactions drug-cell target;
3 -Understanding the processes of cell death;
4- Study of analytical methodologies for the characterization of nucleic acids and proteins;
5 – Critical review of scientific literature relevant for research in the field of expertise.
At the end of this curricular unit, students should be able to:
– Globally understand the cell functioning (eucaryotic cell), know the cellular organelles, identify their relevance and the relationship structure-function; Understand the mode of action of drugs versus the respective cellular targets;
– Choose the analytical molecular technique for a specific purpose;
– Undertake advanced search in databases of scientific literature and critically review it.
Program
MTheoretical contents (T)
A-CELL
B-CELL MEMBRANE
– Organization, function and composition; Membrane specializations; Types of transport; Membrane transporters as drug targets; Drug nanocarriers;
C-CYTOPLASM/CYTOSKELETON
– Structure/function
– Cellular traffic; Signaling
– Cytoskeleton structures as drug targets
D-ENDOMEMBRANE SYSTEMS
– Structure/function; RER and SER; Ribosomes; Golgi apparatus; Lysosomes; Protein synthesis and sorting (UPR and ERAD)
E-MYTOCHONDRIA
F-NUCLEUS
G-CELL DEATH
-Subcellular targets for drug therapy
H-ANALYTICAL METHODOLOGIES IN MOLECULAR BIOLOGY
– Nucleic acid and protein characterization
I-BIOINFORMATICS IN MOLECULAR BIOLOGY
Theoretical-Practical framework (TP)
– ADVANCED DATABASE RESEARCH
-Research, analysis, oral discussion and evaluation of case studies and scientific papers in the field of expertise.
Internship(s)
NAO
Bibliography
Azevedo C, Sunkel CE (2012). Biologia Celular e Molecular, 5ª edição, LIDEL
Alberts, B. et al. Fundamentos de Biologia Celular, ed. Artmed
Alberts, B. et al. Essential Cell Biology, ed. Garland Science
Lodish et al. Biologia Celular e Molecular, ed. Artes Médicas (PT_Brasil) ou Panaamericana
Lodish H, Berk A, Lawrence S et al. Molecular Cell Biology, Freeman and Co., NY, USA
de Robertis. Biologia Celular e Molecular, ed. Guanabara Koogan S.A., Rio Janeiro, Brasil
Principles and techniques of Biochemistry and Molecular Biology, ed. Keith Wilson & John Walker