Microbiology

The theoretical component, although mainly expository and intending to provide the fundamental information on the approached themes, also aims to promote the intervention of the students as well as the discussion of clinical cases focused in the discussed topics.
In the Laboratorial Practice component, the individual application of the intended laboratory procedures will be outstanded. The observed results will be discussed and assessed in group.
The assessment will be made through:
– Group work (10% weight in the final evaluation of the subject),
– two written tests, frequency (70%) and  a practical test (20%).
A minimum score of 9 is required in each element of assessment.
Assessment by Exam.

The knowledge of Microbiology has nowadays enormous importance in areas of health and environment. Thus,the objectives:
-Identify morphological and physiological characteristics of different groups of microorganisms(prions and
viroids,viruses,bacteria,fungi and parasites)
-Knowing the mechanisms of microbial pathogenesis,laboratory diagnosis emphasizing the species of greatest clinical interest and highlighting the different antimicrobial agents used in their treatment (mechanism of action and efficacy studies)
-Recognize the different antiviral,antibacterial,antifungal and antiparasitic agents
-Identify methods of analysis and development of new compounds for the treatment of microbial infections.
-It is also a learning objective the development of basic skills regarding the handling of biological material, observing the basic rules of safety in a microbiology laboratory, and distinguishing the importance of methodologies for the control and prevention of microbial proliferation

T
1-Introduction
2-Virology
Viral classification
Mechanisms of viral pathogenesis
Main viruses and antiviral agents
3-Bacteriology
Morphology, physiology, biochemical characterization and genetics of bacteria
Pathogenic ability and host-bacterium relationship. Microbiome
Infection and immune response
Main bacterial groups:
– Gram positive cocci, Enterobacteriacceae, Pseudomonas, Gram+ and Gram- Anaerobic bacteria.
Antibacterial agents and resistance phenomena
4 -Mycology
Pathogenesis and uses of fungi
Antifungal agents
5 – Parasitology
– Pathogenesis of parasitic diseases
– Antiparasitic agents
PL
– Biosafety – Laboratory of Microbiology
– Optical microscopy: fresh examination and staining
– Identification tests: rapid, biochemical and molecular
– Preparation of culture media
– Control of microbial growth: sterilization, disinfection and antisepsis
– Microorganisms in the environment: air, surfaces and in the human body
– Methods of determining antibacterial activity

NAO