Biological Tecnologies Laboratory

Basic notions of enzyme kinetics and food technology

Laboratory (PL) classes carrying out experimental works in small groups. In these students are confronted with relevant questions, for assessing the previous preparation for the experimental work, and enhancing the knowledge acquired by the students. After each work conclusion, an oral discussion is performed discussing the theoretical concepts involved, and the obtained results. The written reports are also evaluated.
Continuous evaluation is performed taking into account: (i) Evaluation of Experimental Work: 5 summary reports (A-35%) and oral discussion (B-30%) (ii) Experimental Classroom Assessment: Performance in the laboratory classes (C-15%), (iii) Evaluation of an oral presentation of one work results that the student must carry out at the end of the semester (D-20%). The final classification is obtained by summing the contributions of the various components (0.35xA+0.30xB +0.15xC +0.20xD. The student can’t have more than 2 negative ratings in the Experimental Works assessment

The objectives of the course undergo introducing the student in the field of Biological and Enzyme Technology, with special emphasis on the main biological and enzyme uses in industry, enzyme kinetics, biocatalysts immobilization methods and application in reactors.
It is intended that students acquire the following skills: i) recognize the main biological and enzymatic processes in industry; ii) experimentally determine the Michaelis-Menten model kinetic parameters in both free and immobilized enzymes; iii) experimentally determine the parameters of empirical models for continuous reactors with immobilized enzymes; iv) operate biological processes and enzymatic industrial production; v) implement purification methods of biological compounds; vi) organizing and conducting group work and vii) report the performed work, discuss and analyze the experimental results.

The syllabus consists of experimental work in groups of two students. The experimental work includes the execution of experiments, preparation of the written report, and analysis and discussion of experimental results.
Experimental work to be carried out:
1. Amylase production by Bacillus sp. and starch hydrolysis by the action of the produced enzyme
2. Analysis of the kinetics of free and immobilized amylase in batch reactor
3. Operation in continuous reactors with immobilized biocatalysts
4. Enzymatic processing in cheese production
5. Extraction and characterization of bacterial DNA

• - A classificação final é dada pela fórmula: 0,35xRelatórios +0,30x(Discussão oral) +0,15xDesempenho +0,20x(Apresentação oral) - 100.0%

NAO

Fonseca, M.M., Teixeira, J.A., Reactores biológicos: fundamentos e aplicações, Lidel, Lisboa, 2007

Cabral, J.M.S., Aires-Barros, M.R., Gama, M., Engenharia Enzimática, Lidel, Lisboa, 2003

Lima, N, Mota, M., Biotecnologia: Fundamentos e aplicações, Lidel, Lisboa, 2003

Hartmeier, W., Immobilized biocatalysts: an introduction, Springer-Verlag, 1988

Bon, E.P.S., Ferrara, M.A., Corvo, M.L., Enzimas em Biotecnologia: Produção, Aplicações e Mercado, Editora Interciência Ltda., Brasil, 2008

Rehm, H. J., Reed, G.,«Enzyme Technology» in Biotechnology, VCH, 1987

Messing, R. A., Immobilized enzymes for industrial reactors, Academic Press, 1976