Culture of Plant Cells

Biology

The teaching and learning process is based on the following teaching methodologies in order to achieve the objectives of the curricular unit:

a. Teaching of theoretical classes;

b Teaching of practical classes in the laboratory;

c) In the execution of practical work in the field (harvest of plant material), laboratory (cell culture) and nursery (acclimatization of seedlings obtained by in vitro propagation);

d) In the bibliographic research and analysis of articles and reports on the contents of the CU.

For a better consolidation of the concepts and methods used in the classes (in the laboratory or in the nursery), the students are followed weekly in tutorial orientation classes.

Students are required to attend classes. The minimum attendance value is 75% of the number of classes.

The learning objective of this course unit is to promote, to the student who successfully completes it, the following competences identified below.

1. The student knows the techniques applied in plant cell cultures and their applications in breeding and conservation of genetic resources.

2. The student identifies methods for medium- and long-term conservation. The student knows the techniques applied in plant cell cultures and their applications in increasing genetic variability and in conservation and evaluation of plant germplasm.

The acquisition of skills are obtained through the following learning outcomes:

The acquisition of competences in Module 1 are obtained through the following learning outcomes:

a Identifies the influence of plant material and physical factors on the growth and development of crops.

b Identifies the various types of vegetative multiplication in vitro.

c Identifies the various stages of development to your specific requirements.

The acquisition of competences in Module 2 are obtained through the following learning outcomes:

a Identifies the different methods of conserving plant cultures in vitro.

b Identifies the different methods for evaluating the efficiency of in vitro plant crop conservation techniques.

The syllabus contents to achieve the two learning objectives described are divided into two learning modules:

Module 1: Plant cell culture and its applications in plant breeding.

Module 2: Plant cell culture and its applications in the conservation of genetic resources.

• - Módulo 2 ponderação de 35 %. 1) testes (85%); 2) avaliação trabalhos práticos (15%). - 35.0%
• - O Módulo 1 ponderação de 65 %. Módulo 1: 1) testes (75%); 2) trabalhos práticos (10%); 3) apresentação artigo (15%). - 65.0%

NAO

CANHOTO, J.M. – Biotecnologia vegetal, da clonagem de plantas à transformação genética. Imprensa da Universidade de Coimbra. 2010. ISBN 978-989-26-0065-9.

ENGELMANN, F. – Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell.Dev.Biol.-Plant. DOI 10.1007/s11627-010-9327-2. Vol: 47, 2010, 5-16.

GEORGE, E.F., DE KLERK, G.J. The components of plant tissue culture media I: macro and micro-nutrients. In Plant propagation by tissue culture, 3rd ed. (eds. George, E.F., Hall, M.A. & De Klerk, G.J.), Springer, Dordrecht. ISBN 978-1-4020-5004-6, 2008, pp. 65-114.

OZUDOGRU, E.A., PREVIATI, A., LAMBARDI, M. In Vitro Conservation and Cryopreservation of Ornamental Plants. In Protocols for in Vitro Propagation of Ornamental Plants, Methods in Molecular Biology, Jain, S.M. & Ochatt, S.J. (eds.), vol. 589, DOI 10.1007/978-1-60327-114-1_28, © Humana Press, LLC, 2010, pp. 303-324.

PREECE, J. – Stock plant physiological factors affecting growth and morphogenesis. In: Plant propagation by tissue culture, 3rd ed., George, E.F., Hall, M.A., De Klerk, G.J. (Eds.), Springer, Dordrecht. ISBN 978-1-4020-5004-6, 2008, pp. 403-422.

PUROHIT, S.D.-. Introduction to plant cell tissue and organ culture. PHI Learning Private Limited. Delhi, 2012, ISBN 978-81-203-4677-2.