Bioreactors and Biocatalysis

Base Knowledge

Is recommended prior knowledge of enzyme and microbial kinetics as well as biochemical engineering.

Teaching Methodologies

Theoretical and practical classes. Theoretical contents explanation. Solving exercises. Laboratorial practices in bioprocesses: up-stream, control and down-stream processes. Be critical in the application of theoretical knowledge and in the selection of relevant information related to the addressed issues.

Learning Results

This course aims to address bioprocesses issues in a more complex and integrated approach of microbial and enzymatic kinetics, energy and mass transfer phenomena and unit operations applied to systems and products manufactured by this means.
Is intended that students recognize the bioreactor as the fundamental element of bioprocesses. Understand the essential mechanisms in the operation and design of complex bioreactors. Collect and process information about bioproducts and bioprocesses to: select the bioreactor operating mode and configuration? dimension the bioreactor mixing, aeration and heat transfer systems? identify downstream processes for the recovery of a specifc product.


1. Production of add-value compounds by using bioreactors.
2. Bioreactors technology. Product formation in microbial culture (growth-linked and non growth-linked products) and substrate consumption. Enzymatic reaction kinetics. Types of bioreactors, operation mode and process configurations (e.g. chemostat culture with recycle? airlift, multi-stage systems? heterogeneous systems). Mixture, agitation and aeration. Mass and heat transfer in sterilization processes. Scale-up and scale-down processes.
3. Bioproducts separation and purification. Solid-liquid separation. Concentration. Cell disruption (mechanical and non mechanical methods).
4. Conventional and nonconventional applications of microbial and enzymatic bioprocesses: case studies.

Grading Methods

Avaliação Contínua
  • - Componente Prática = Desempenho prático * 20% + Trabalhos Práticos (relatório+apresentação+defesa) * 80% - 40.0%
  • - Componente teórica (teste escrito) - 60.0%
Avaliação Por Exame
  • - Exame Escrito - 100.0%




Fonseca MM e Teixeira JA (2007). Reactores Biológicos – Fundamentos e Aplicações, Lidel.

Doran PM (2004) Bioprocess Engineering Principles. Elsevier Academic Press.

Stanbury P, Whitaker A, Hall SJ (1995) Principles of Fermentation Technology, 2nd edition, Butterworth-Heinemann.

Shuler ML and Kargi F (1992). Bioprocess Engineering, Basic Concepts. Prentice Hall.

Bailey JE (1986). Biochemical Engineering Fundamentals, McGraw Hill.