Base Knowledge
Anatomy, Histology and Histotecnology
Teaching Methodologies
The theoretical classes are aimed at the detailed presentation of the contents. Use an expository -active methodology, lectures, presentations with discussion and debate.
The practical part of curricular unit focuses on the view of blades, as well as the elaboration of a cytopathological report.
Learning Results
Differentiate the procedures of a technical nature for different types of cytological material.
– To identify cell patterns for respiratory cytology, breast aspiration cytology, thyroid aspiration cytology, ganglion aspiration cytology.
– Know the application of immunocytochemistry, flow cytometry and molecular biology techniques in exfoliative cytology and aspiration puncture.
– Carry out the screening with the interpretation and preparation of the respective report.
Program
RESPIRATORY CYTOLOGY
Introduction to cytological technique and normal cytology in respiratory specimens
Inflammatory, infectious and non neoplastic changes
Lung Neoplasias
Iatrogenic changes
Cytopathological report
New Ratings
ASPIRATION CYTOLOGY
Technical procedures on FNA
Aspiration punctures ultrasound guided and ecoendoscopy
BREAST ASPIRATION CYTOLOGY
Normal cytology
Inflammatory changes
Benign and malignant primary pathology
Metastatic tumours
New Ratings
ASPIRATION CYTOLOGY OF THYROID
Normal cytology
Inflammatory changes
Benign pathology and primary and secondary neoplastic
Bethesda classification
ASPIRATION CYTOLOGY OF LYMPH NODE
Normal and reactive inflammatory cytology
Primary and metastatic neoplastic pathology
importance of techniques in immunocytochemistry, flow cytometry and molecular biology in cytopathological diagnosis.
PRACTICE
– Understanding of the pre analytical phase. Concepts and examples:
– Collection and transport of samples
– Requirements and registrations. Care to be taken.
– Execution of smears in exfoliative cytology.
– Simulation of aspiration puncture collection and swab execution.
-Staining Techniques: Papanicolaou, Giemsa and Hematoxylin Eosin.
-Execution of imprint.
-Execution of conventional “Cell Block”.
– Execution of the “Cell Block” from the remaining material of cytology in liquid based.
-Microscope evaluation for quality control
-Screening of the theoretical material samples: lung, thyroid, breast and lymph node.
Curricular Unit Teachers
Internship(s)
NAO
Bibliography
Primary References:
1. Cibas, E.D. (2010). The Bethesda System for Reporting Thyroid Cytopathology- definitions, criteria and explanatory notes
2. Mckee, G.T. (1997). Cytopathology. London:Mosby-Wolfe
3. Koss, L.G. (2006). Diagnostic Cytology and its histopathologic bases.5th Edition.
Philadelphia:J.B.Lippincott.Vol.I e II
4. Keebler C. M. Somrak T.M. (1993). The manual of Cytophatology. 7th Edition.USA: ASCP
5. Kurman R.J., Solomon D. (2004). THE BETHESDA SYSTEM for reporting cervical/vaginal cytology
diagnosis – definitions, criteria and explanatory notes for terminology and specimen adequacy. New York, Inc. Springer – Verlag
6. Wield, G. L. Keebler C.M., KossL. G., Patten S. F., Rosenthal D.L. (1992). Compendium on Diagnostic Cytology. 7th Edition. Chicago, Illinois, USA: Tutorial of Cytology
Secondary References:
Atkinson B.F., Silverman J.F. (2000) Atlas de dificuldades diagnósticas em citopatologia:Madrid:Harcout