Clinical-Laboratorial Biochemistry II

General Biochemistry, Physiology, Cell Biology.

The theoretical classes are aimed at the detailed presentation of the program contents. Use an expositoryactive methodology, appealing the direct intervention of the students. In the practical part of the curricular unit it’s privileged the execution of practical laboratory assignments, explanations and discussion of clinical cases.

Theoretical and practical fundamentals of Clinical-Laboratorial Biochemistry (CLB);

• Analytical techniques for the determination of the analytical parameters adjusted to its objectives;

• Understand the fundamental concepts of analytical methods studied will allow qualitative and/or quantitative analytical conclusions;

• Handle in a scientifically correct, expeditious and safe way the laboratory instrumentation, as well as the implementation of the analytical techniques with view to obtain more accurate and secure results as possible;

• Select the most appropriate methodology to solve a problem of laboratory analysis, taking into account specific requirements like detection sensitivity, specificity and reproducibility among others;

• Interpret critically the results and assess their quality;

• Develop capacities on a teamwork level;

• Practical use of instrumental methods of analysis more common in CLB;

Iron metabolism:

– Synthesis of porphyrins and heme;

Catabolism of heme and bilirubin synthesis:

– Laboratory evaluation and associated pathologies.

Metabolism of purines and pyrimidines:

– Uric acid synthesis;

– Laboratory evaluation and associated pathologies.

Lipid metabolism:

-Laboratory evaluation and associated pathologies.

Biochemical analysis of fluid extravascular :

-Laboratory evaluation and its importance in the diagnosis and/or monitoring.

Thyroid and parathyroid gland:

-Calcium metabolism;

Adrenal glands:

-Laboratory evaluation and associated pathologies.

Neoplasias and tumour markers:

-Laboratory Assessment

Vitamins and trace elements:

-Laboratory evaluation and associated pathologies.

The clinical laboratory in the monitoring of drugs therapeutic and/or abuse.

Collection of biological samples for analytical determinations.

NAO

Primary bibliography:

Ahmed, Nessar, ed. lit. – Clinical biochemistry. Second edition. Oxford : Oxford University Press, cop. 2017.

Burtis, CA; Bruns, DE, Sawyer, BG, Tietz, NW. Tietz fundamentals of clinical chemistry and molecular diagnostics. seventh edition. ed. St. St. Louis, Mo: Elsevier/Saunders, cop. 2015.

Gaw, Alan et al – Clinical Biochemistry: An Illustrated Colour Text. 5th ed. Churchill-Livingstone Elsevier, 2013.

Kaplan, Lawrence; Pesce, Amadeo – Clinical Chemistry: Theory, Analysis, Correlation. 5th ed. St. Louis, Mo.: Mosby/Elsevier, 2010.

Secondary bibliography:

Murphy, Michael, et al – Clinical Biochemistry: An Illustrated Colour Text. 6th ed. Churchill-Livingstone Elsevier, 2019.

Rifai, N., et al., Tietz fundamentals of clinical chemistry and molecular diagnostics. Eighth edition. ed. St. Louis, Missouri: Elsevier, 2019.

Bishop, M.L., E.P. Fody, and L.E. Schoeff, Clinical chemistry : principles, techniques, and correlations. Eighth edition. ed. Philadelphia: Wolters Kluwer, 2018.

Mcpherson, Richard; Pincus, Matthew; Henry, John – Henry’s Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia: Saunders Elsevier, 2011.

Devlin, Thomas M – Textbook of Biochemistry with Clinical Correlations. 7th ed.: Wiley, 2010.