Cytopathology I

Base Knowledge

Anatomy, Histology, Histotecnology

Teaching Methodologies

In the theoretical classes there will be a detailed presentation of the program contents, using an expository, interrogative and active methodology with direct student’s participation.

In the practical classes will be use a demonstrative methodology of the concepts learned, with resolution and discussion of pratical problems,  implementation of individual techniques and microscopic observation of histological slides.

Learning Results

– Differentiate technical procedures for different kinds of cytological material.

– Identify cellular patterns for respiratory cytology and gastro intestinal tract.

– Perform the screening with the interpretation and elaboration of the respective report.

Program

Gynecological Cytology

Technical Procedures

Normal cytology

Artifacts and contaminants

Hormonal Variations

Female genital tract physiology

Reactive and inflammatory changes

Microbiology

Iatrogenic changes

Intraepithelial lesions: pathogenesis of cervical neoplasia

Squamous Carcinoma

Glandular neoplasms

Extrauterine Tumors

Endometrium

Pitfalls in vaginal cervical cytology

Bethesda classification

Screening Programs

Preparation of cytological report

 

Cytology of Spills

Serous Cavity Liquid Collection Technique

Anatomy and physiology

Processing Techniques

Normal cytology

 

Nonmalignant diseases. Inflammatory processes

Metastatic tumors and lymphomas

Mesothelioma

Iatrogenic changes Immunocytochemical markers

Preparation of cytological report

New Classification

Urinary Cytology

Cytological Technique

Anatomy and Histology

Normal cytology

Inflammatory and infectious changes

Malignant Neoplasms

Iatrogenic changes

Ileal bladder

Paris classification

Preparation of cytological report

 

Practice

– Understanding of the pre analytical phase. Concepts and examples:

– Collection and transport of samples

– Requirements and registrations. Care to be taken.

– Execution of smears in exfoliative cytology.

– Simulation of aspiration puncture collection and swab execution.

Staining Techniques: Papanicolaou, Giemsa and Hematoxylin Eosin.

-Execution of imprint.

-Execution of conventional “Cell Block”.

– Execution of the “Cell Block” from the remaining material of cytology in liquid medium.

-Microscope evaluation for quality control

-Screening of samples of the material studied in the theoretical: cervicovaginal cytology, strokes and urine.

Curricular Unit Teachers

Internship(s)

NAO

Bibliography

Primary References:

– Junqueira LC, Carneiro J. (1997)Noções básicas de citologia, histologia e embriologia. 9º ed

 Referências Secundárias:

– Mckee, G.T. (1997). Cytopathology. London:Mosby-Wolfe

– Koss, L.G.(2006). Diagnostic Cytology and its histopathologic bases.5th Edition. Philadelphia:J.B.Lippincott.Vol.I e II

– Keebler C. M. Somrak T.M.(1993). The manual of Cytophatology. 7th Edition.USA: ASCP

– Kurman R.J., Solomon D. (2004). THE BETHESDA SYSTEM for reporting cervical/vaginal cytology diagnosis – definitions, criteria and explanatory notesd for terminology and specimen adequacy. New York, Inc. Springer – Verlag