Base Knowledge
Biology, Genetics, Molecular Biology
Teaching Methodologies
1. Presentation by the teacher of content related to the theoretical aspects of this curricular unit.
2. Bibliographical research on some topics covered in this UC;
3. Execution of practical laboratory work related to gene manipulation and transformation techniques;
4. Preparation of reports related to different topics
For a better consolidatios of concepts, students are accompanied weekly in tutorial classes. Students are required to attend classes. The minimum attendance rate for each type of class is 75% of the number of classes taught
Learning Results
1. Know the relevant techniques in recombinant DNA technology and its insertion and expression in bacteria and yeast. 2. Apply gene manipulation techniques to plants. 3. Know and apply genetic manipulation in animal cells.
Program
Module 1. Concepts about Genetic Engineering; understand genes, genomes and “-omes”; gene manipulation methods; Genetic engineering applied to plants;
Module 2. Most common genetic recombination techniques; Host cells and cloning vectors; Cloning strategies; selection and analysis of recombinants; genetic technology applied to microbial and animal improvement.
Curricular Unit Teachers
Grading Methods
- - Modulo 1: 1) teste escrito (70%); 2) relatórios dos trabalhos práticos (15%); 3) apresentação e discussão de um tema (15%) - 60.0%
- - Modulo 2: 1) teste escrito (75%); 2) participação nos trabalhos práticos (25%). - 40.0%
Internship(s)
NAO
Bibliography
Avise, John, C. The Hope, Hype and Reality of Genetic Engineering. Oxford University Press, USA, (2004) pp 242.
Brown, T.A.Gene Cloning & DNA Analysis. An Introduction. 5th. Ed. Blackwell Publishing, UK, (2006) pp 386.
Jones and Bartlett Publishers, USA, (2003) pp 562. Khachatourians, G.G., McHughen, A., Scorza, R., Nip, W. and Hui, Y.H. Transgenic Plants and Crops. Marcel Dekker, Inc., New York, USA, (2002) pp. 876.
Lima, N, & Mota, M. Biotecnologia. Fundamentos e Aplicações. LIDEL, Lisboa, Portugal, (2003) pp. 505.
Malacinski, G.M. Fundamentos de Biologia Molecular. 4ª Ed. Guanabara Koogan S.A., Brasil, (2005) pp. 439.
Neal, C. Stewart, Jr. Genetically Modified Planet. Environmental impacts of genetically engineered plants. Oxford University Press, USA, (2004) pp 240.
Nicholl, D.S. An Introduction to Genetic Engineering. 2nd Ed. Cambridge University Press, UK, (2005) pp. 292.