Histochemistry and Immunocytochemistry

Anatomy, Cellular Biology and Histology.

In the theoretical classes, there will be a detailed presentation of the program contents, using an expository, interrogative and active methodology with direct student’s participation.

In the practical classes will be use a demonstrative methodology of the concepts learned, with resolution and discussion of practical problems, implementation of individual techniques and microscopic observation of histological slides.

The student must acquire knowledge about:

1. Physico-chemical principles of histochemistry.

2. Dye classification and stain classification.

3. Identification of histochemical stains for different tissue components.

4. Basic principles of immunocytochemistry.

5. Analytical and pre-analytical factors that affect immunostaining.

6. Immunomarking methods.

7. Quality control in immunohistochemistry.

The student must acquire skills to:

1. Program and perform the Histochemical and Immunohistochemical methods complementary to the diagnosis.

2. Select and prepare solutions, stainings and dilutions of antibodies.

The student must acquire skills to:

1. Apply and control histochemical and immunohistochemical methodologies in a biomedical context, taking into account the cellular or tissue target.

2. Plan and control the histochemical staining and immunostaining procedures

3. Know the application of histochemistry and immunohistochemistry in the diagnosis / prognosis of pathologies and in targeted therapies

Theoretical:

– General concepts of Histochemistry;

– Types of dyes and stainings;

– Characterization of staining techniques for connective tissue, nervous tissue, carbohydrates, lipids, amyloid, microorganisms and pigments and minerals;

– General concepts of Immunocytochemistry;

– Principle and methods for antigenic recovery;

– Immunostaining methods;

– Controls in immunocytochemistry;

– Main antibody panels: colorectal, breast, lung and lymphoproliferative tumors.

Practical:

– Perform histochemical techniques studied in theoretical classes.

– Perform and optimize immunohistochemistry protocols

NAO

Primary References:

1. Bancroft, J. & Gamble, M. (2002). Theory and Practice of Histological Techniques (5.ªed.). London: Churchill Livingstone.

2. Moral, Raimundo Garcia del (1993). Laboratório de Anatomia Patológica (1ª ed.). Madrid: McGraw-Hill – Interamericana de Espana.

3. Carson, Freida (2000). Histotechnology: A Self-Instrumentation Text (2ª Ed.). American Society Clinical Pathology  

Secondary References:

– Luna, L. (1992). Histopathologic Methods and Color Atlas of Special Stain and Tissue Artifacts. Maryland: Ameerican Histolabs.

– Kiernan, J.A. (2208) Histological and histochemical Methods (4th Ed). England:Scion Publishing Ltd ISBN:9781904842422.