Laboratory Methods and Techniques

General chemistry.

The theoretical classes are aimed at the detailed presentation of the program contents. Use an expositoryactive methodology, appealing the direct intervention of the students, favouring openly an abundant iconography instead of the detriment of a theoretical-descriptive teaching.

In the practical part of curricular unit its privileged the execution of practical work in laboratory, explanations and demonstrations using computer programs, multimedia display and resolution of exercises.

-Theoretical fundaments underlying the instrumental methods discussed, as well as the function of the main components of the instruments used, its limitations and potentialities;

-Biomedical applications underlying the electrochemical methods, immunological, spectroscopic,

chromatographic and electrophoretic;

-Understand the fundamental concepts of analytical methods studied in order to be able to draw analytical qualitative and/or quantitative conclusions;

-Handle in a scientifically correct, expeditious and safe way the laboratory instrumentation;

-Select the most appropriate methodology to solve a problem of laboratory analysis, taking into account specific requirements as detection sensitivity, specificity and reproducibility among others;

-Interpret critically the results and assess their quality;

-Develop capacities at the level of teamwork;

-Practical use of instrumental methods of analysis more common in clinical laboratory;

-Introduction to laboratory methods and techniques: understanding of analytical accuracy, calibration curves.

-Immunological Methods: theoretical foundations, generic applications and biomedical applications; types of immunological tests.

-Electro analytical Methods: theoretical foundations, generic applications and biomedical applications; potentiometry; conductimetry; coulometry.

-Spectroscopic Methods: theoretical foundations, generic applications and biomedical applications; molecular absorption spectrometry; atomic absorption spectrometry; flame emission spectrometry; nephelometry; turbidimetry; mass spectrometry; nuclear magnetic resonance.

-Chromatographic and electrophoretic separation methods: General principles, generic applications and biomedical applications; SDS-PAGE electrophoresis; two-dimensional electrophoresis; capillary electrophoresis; western blotting; thin layer chromatography; column chromatography; hyphenated techniques.

NAO

Primary bibliography:

Kaplan, Lawrence A., and Amadeo J. Pesce. Clinical Chemistry : Theory, Analysis, Correlation. 5th ed. St. Louis, Mo.: Mosby/Elsevier, 2010.

Rouessac, Francis, and Annick Rouessac. Chemical Analysis : Modern Instrumentation Methods and Techniques. 2nd ed. Chichester, England ; Hoboken, NJ: John Wiley, 2007.

Skoog, D.A., Holler, F.J., Crouch, S.R. – Principles of instrumental analysis. Sixth edition. Australia: Thomson, cop. 2007.

Secondary bibliography:

Skoog, D.A., F.J. Holler, and S.R. Crouch, Principles of instrumental analysis. Seventh edition. ed. Australia: Cengage Learning, 2018.

Skoog, Douglas A. Fundamentals of Analytical Chemistry. 9th Ed. ed. Belmont, CA: Cengage – Brooks/Cole, 2012.

McPherson, Richard A., Matthew R. Pincus, and John Bernard Henry. Henry’s Clinical Diagnosis and Management by Laboratory Methods. 21st ed. Philadelphia: Saunders Elsevier, 2007.