Microbiology and Immunology

Biology

To achieve the objectives of the course unit and the attainment of the expected competences to the students, the teaching-learning process is based on

1) Expositive theoretical lessons, with audio-visual and “power point” accompaniment and also, with articles discussion and thematic research presentation (T), allowing the professor to evaluate the student progress in the knowledge acquisition;

2) Practical laboratory classes with execution of demonstrative techniques.

3) Tutorial classes in which the acquisition of knowledge and the execution of works by the student involves the close collaboration of the teacher, preferentially with case studies applied to animal immune system response.

MODULE A:

1. Know the principles of taxonomy and the morphological and cultural characteristics of the main microbial groups;
2. Recognize the sources of contamination and factors influencing the growth of microbial populations; Know mechanisms to control microorganisms (disinfection and sterilization)
3. Develop the ability to work in the laboratory in aseptic conditions and prepare and sterilize all necessary material; Know the methods for quantitative assessment of microbial populations and general techniques for isolation, repiculation and maintenance of microbial strains;

MODULE B:

4. Recognize the role of the immune system in the body’s defense;
5. Explain the constituents and processes that figure in the immune response;
6. Explain the mucosal immunity and the vaccination process.

MODULE A:

1. taxonomy and morphological and cultural characteristics of the main microbial groups; prokaryotes and eukaryotes; 2. cultural and biochemical characterization; Gram staining, catalase and oxidase test; population growth of a microbial culture; growth curves; characterization of microorganisms; 3. microbial control and handling in asepsis; characterization and preparation of culture media; isolation and purification techniques of microbial strains.

MODULE B:

4. role of the immune system: characteristics of the immune response. Non specific response (natural barriers, phagocytosis, NK, neutrophils); Specific response (T and B lymphocytes). 5. constituents and processes in immune response: lymphoid organs, cells and cytokines. Origin, circulation, localization and functions of cells. Antigen processing and presentation and MHC molecules. 6. 6. mucosal immunity. Th1 and Th2 type responses. Vaccines and vaccination: antigens and adjuvants. Primary and secondary response; applications to the diagnosis.

NAO

Current Protocols in Immunology (Wiley, 2018). [E-Reader Version]. Online ISBN: 9780471142737. DOI: 10.1002/0471142735. Retrieved fromhttps://currentprotocols.onlinelibrary.wiley.com/loi/1934368.

Ferreira, Wanda F.C.e SOUSA, J.C.F. (2000). Microbiologia , Vol 2. Lisboa: Edições Técnicas Lda.

Ferreira, Wanda F.C.e SOUSA, J.C.F. (2002). Microbiologia , Vol 3. Lisboa: Edições Técnicas Lda.

Ferreira, W.F.C. & Sousa, J.C.F. & Lima, N. (2010). Microbiologia. Lidel-Edições técnicas, Lda. Lisboa. 622 páginas. ISBN 978-972-757-515-2.

Gerald N. Callahan, Robin M. Yates (2014). Basic Veterinary Immunology. University Press of Colorado, p.337. ISBN 1607322188, 9781607322184.

Mcvey, S., Kennedy, M., Chengappa, M. (2016). Microbiologia Veterinária.

Guanabara Koogan, 3ª edição. ISBN 9788527728263.

Quinn, P. J. ;Markey, B. K. ; Cater, M. E; Donnely, W. J. ; Leonar, F. C.(2005). Microbiologia Veterinária e Doenças Infecciosas. 1ª Ed, Artmed. 512 páginas ISBN: 9788536304861.

Sousa Dias, Carlos Dias Pereira, Isabel Herder, Joaquim Soares, Maria Antónia Conceição, Micaela Soares. Manual de Higiene e Sanidade Animal. Ministério da Agricultura do Desenvolvimento Rural e das Pescas, República de Angola. Projecto SINFIC com Huambo (Angola) – (curso profissional Agro-pecuária).

Yuan, S., Tao, X, Huang, S., Chen,S., Xu, A. (2014). Comparative Immune Systems in Animals. Annual Review of Animal Biosciences. Vol. 2:235-258.

https://doi.org/10.1146/annurev-animal-031412-103634.