Base Knowledge
Not applicable.
Teaching Methodologies
The basic notions of this curricular unit will be covered through expository projection in PowerPoint, with the resolution of worksheets to assess the acquired knowledge. Additionally, the practical application of these concepts will be carried out through practical experiments to identify cell morphologies and structures. The study material is available through the platform adopted by ISEC.
Learning Results
The main objective of this curricular unit is to provide students with the fundamental concepts of Cellular Biology, so that they are able to understand the concepts covered in subsequent courses in the degree curriculum, in the area of Biological Sciences.
The aim is for students to be able to: i) recognize the principles of microscopy and know how to use the microscope; ii) identify and distinguish cellular structures and functions in prokaryotic and eukaryotic cells; iii) recognize the structure of the main animal tissues; iv) apply appropriate histological and staining techniques.
Program
1. The study of the cell
Fundamentals of optical and electron microscopy. Main applications of microscopy. Main simple and differential stains used in Cell Biology.
2. Structure of prokaryotic cells
The prokaryotic and eukaryotic cell. Morphological characteristics of prokaryotic microorganisms. Cellular structures and function in prokaryotic cells. Prokaryotic latent forms.
3. Structure of eukaryotic cells
Cellular structures and function in eukaryotic cells. Eukaryotic latent forms.
4. Introduction to animal tissue biology. Histological processing and staining.
PRACTICE
Carrying out visualization and staining experiments using optical microscopy. The work to be carried out includes the preparation of slide and smear samples, negative staining with methylene blue, Gram, Sudan black (PHA granules) and malachite green (endospores).
Curricular Unit Teachers
Internship(s)
NAO
Bibliography
1. Azevedo, C. (2005). Biologia Celular e Molecular. Lisboa: Lidel.
2. Robertis, E., Robertis Jr., E. M. (2005). Biologia Celular e Molecular. Lisboa: Fundação Calouste Gulbenkian.
3. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2002). Molecular Biology of the Cell. New York: Garland Science.
4. Pelczar, M.J., Chang, E.C.S., Krieg, N.R. (1996). Microbiologia: conceitos e aplicações. São Paulo: Makron Books.
5. Willey, Sherwood, Woolverton (2008). Prescott, Harley and Klein’s Microbiology. Boston: McGraw Hill.
6. Black. G. (2005). Microbiology: principles and explorations. New Jersey: John Wiley & Sons.
7. Ferreira, Sousa, Lima. (2010). Microbiologia. Lisboa: Lidel.
8. Junqueira e Carneiro. (2008). Histologia Básica. Guanabara Koogan.
9. Ters e Kierszzenbaum (2021). Histologia e Biologia Celular – Uma introdução à patologia. Guanabara Koogan.
10. Michael J. Leboffe; A Photographic Atlas of Histology, Morton Publishing Company, 2013.