Molecular Applied Biotechnology

Biology
Molecular Biology
Chemistry

To achieve the objectives of the course and to provide the foreseen skills to students, the teaching learning
process is based on:
1. lectures focussing the theoretical aspects of this course?
2. guided literature research on methods and techniques used in the field of Applied Molecular Biology?
3. laboratorial work proposed by the teacher.
The classification of the CU is obtained by the weighted average of theoretical and practical examination marks.

Reviewing knowledge that constitute the molecular basis for the practice of Biotechnology.
Understand that genomics, proteomics and metabolomics are the basis of Biotechnology.
Explain how the study of molecular biology enables the development of tools for fundamental and applied research, including recombinant DNA technology.
Run laboratory protocols related to Molecular Biology techniques.

Molecular organization of the microbial genome: nucleic acids, genes and chromosomes. Model organisms in molecular biology.
Cell cycle and its regulation? replication, mutation and DNA repair.
Gene expression and its regulation in prokaryotes and eukaryotes.
Mechanisms to generate variability: mutations and bacterial recombination.
Improvement of industrial strains: classic and directed mutagenesis.
Expression of heterologous proteins: cloning and expression vectors, microbial host cells.
Application of molecular biology techniques in industrial microbiology.
Genotyping of microorganisms: techniques, tools and methodologies based on PCR, hybridization and DNA sequencing.

Avaliação Contínua

• - Teste escrito teórico-prático - 100.0%

Avaliação por Exame

• - Teste escrito teórico-prático - 100.0%

NAO

Lewin, B. Genes IX. 9.ª ed. Sudbury, Mass.: Jones and Bartlett Publishers 2007.

Base geral de artigos científicos: http://www4.ncbi.nlm.nih.gov/PubMed/

Nicholl, D.S.T. An Introduction to Genetic Engineering. Cambridge University Press, Cambridge. 1994.

Livros e jornais http://163.238.8.180/~davis/Bio_327/molbio_links.htm

Lodish, H., Berk, A., Matsudaira, P., Kaiser, C.A., Krieger, M., Scott, M.P., Zipursky, S.L. & Darnell, J. Molecular Cell Biology. 5ª ed. W.H. Freeman and Company, USA. 2004.

Dicionário de Biologia Celular e Molecular: http://www.mblab.gla.ac.uk/dictionary/graphic.html

Smith, C.A. & Wood, E.J. Molecular Biology and Biotechnology. 1ª ed. Chapman and Hall, Londres. 1991.

Brown, T.A. Gene Cloning and DNA Analysis: An Introduction. 5.ª ed. Blackwell Science Inc, Londres 2006.

Hartl, D. & Jones, E. Genetics. Analysis of Genes and Genomes. 6ª ed. Jones and Bartlett Publishers, Sudbury. 2005.

Griffiths, A.J.F., Wessler, S.R., Lewontin, R.C., Gelbart, W.M., Suzuki, D.T. & Miller, J.H. Introduction to Genectic Analysis. 8th ed. W. H. Freeman and Company, Nova Iorque. 2005.

Sambrook, J. & Russell, D. Molecular Cloning. A Laboratory Manual. 3ª ed. Cold Spring Harbor Laboratory, Nova Iorque. 2001.

Arraiano, C.M. & Fialho, A.M. O mundo do RNA. 1ª. ed. Lidel, Lisboa. 2007.

Videira, A. Engenharia Genética – Princípios e Aplicações. Lidel, Lisboa. 2001.