Analytical Chemistry In Pharmacy I

General inorganic chemistry; Inorganic chemistry

Expository theoretical classes using audiovisual and interactive means between student and teacher, using theoreticalpractical classes to reinforce the understanding of theoretical syllabus and its application in the laboratory matrix.
Two hours a week are available, with a pre-defined time or to be arranged with the student to clarify doubts.
Each student is also given the pedagogical support of all the material taught or the bibliographical references
recommended for the discipline, at the beginning of the semester.
Continuous assessment: Theoretical and practical attendance
Students will have to obtain an assessment equal to or greater than 10 in practical frequency and 9.5 in theoretical frequency.

Theoretical part and theoretical-practical 55%; practical part 35%; attendance 10% – the presence, punctuality and active participation of students in classes are valued in the final calculation of the continuous assessment.

Students are admitted to the exam, taking into account the specifications of the Academic Regulation.

The curricular unit of Analytical Chemistry in Pharmacy I aims to provide the student with an adequate knowledge of theoretical principles, qualitative and quantitative analysis, practical components and application of classical methods and instrumental methods of analysis, indispensable to the performance of laboratory activities, with the main objective of applying new technologies to the field of Pharmacy, its validation, quality control, as well as in the area of Pharmaceutical Industry and research. Knowledge of GLP, GMP and ICH systems in the analysis of medicines in quality control, as well as compliance with quality specifications, identification, purity, dosage; role of Pharmacopoeia monographs and reference substances.

Theoretical matrix
Theoretical framework
1. INTRODUCTION TO ANALYTICAL CHEMISTRY – 2 hours
1.1. Qualitative analysis: chemical processes, physical, physico-chemical and thermal
1.2. Quantitative analysis: chemical processes, physical and physicochemical
1.3. Analytical process
1.4. Classical analytical methods and instruments
1.5. Comparison of analytical methods
2. ERRORS IN ANALYTICAL CHEMISTRY – 2 hours
2.1.Types of errors
2.2. Accuracy and precision
2.3. Statistical analysis of experimental data
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2.4. Mean and standard deviation
2.5. Confidence interval and reliability testing
3. QUALITY CONTROL AND QUALITY SYSTEMS analytically 2hours
3.1 Quality definition
3.2 Quality requirements
3.3 Quality control system
3.4. Statistical quality control
3.5. The 7 Traditional Tools
3.6 Quality management
3.7 Quality assurance
4. ACCREDITATION AND CERTIFICATION of LABORATORIES-2h
4.1 Certification
4.2 Accreditation
5. METHODS CALIBRATION – 2 hours
5.1. Calibration curves
5.2. Standard solutions
5.3. Linear regression
5.4. Standard addition method
5.5. Internal standard
6. SAMPLING – 2 hours
6.1. Sampling plan
6.2. Sampling Techniques
6.3. Sampling Procedures
6.4. Sampling records
7. LABORATORY AUTOMATION – 2 hours
7.1. Continuous analyzers
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7.2. Discontinuous analyzers
8. VALIDATION OF METHODS – 2 hours
8.1. Standardized method
8.2. Internal method
8.3. Validation by indirect evaluation
8.3.1. Practicability
8.3.2. Calibration curves
8.3.3. Working Range
8.3.4. Linearity
8.3.5. Detection limit of the method
8.3.6. Quantification limit of the method
8.3.7. Sensibility
8.3.8. Accuracy / Loyalty
8.3.9. Specificity / Selectivity
8.3.10. Robustness
8.4. Validation by direct assessment
8.4.1. Certified reference materials
9. VOLUMETRIC METHODS – 6 hours,
9.1. Types of volumetric methods and evaluation of the titration solutions
9.2. Acid-base titrations
9.2.1. Titration of acids and strong and weak bases
9.2.2. equivalence point detection methods
9.2.3. Applications with analytical interest
9.3. Oxidation-reduction titrations
9.3.1. Reactions with electron transfer
9.3.2. Methods of detection of the equivalence point in redox reactions
9.3.3. Features and reactions of some titrants used in redox volumetry
9.3.4. Applications with analytical interest
9.3. Complexing titrations
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9.3.1. complexation reactions; complexing agents
9.3.2. equivalence point detection methods
9.3.3. Applications with analytical interest
9.4. Precipitation tritation
9.4.1. precipitation reactions; solubility
9.4.2. equivalence point detection methods
9.4.3. analytical applications
10. METHODS gravimetric – 2 hours
10.1. General characteristics of gravimetric methods
10.2. Precipitation
10.3. Formation of a precipitate: nucleation, crystallization and aging
10.4. Nucleation and crystal growth
10.5. Colloids
10.6. Contamination of precipitates
10.7. analytical applications
11. ELECTROCHEMICAL METHODS – 6 hours,
11.1. General principles
11.2. Types of electrochemical cells
11.3. Potential electrode (Nernst equation)
11.4. Potentiometry
11.4.1. Basic principles and instrumentation
11.4.2. Types of electrodes; indicator electrode, reference membrane and combined
11.4.3. Direct potentiometric
11.4.4. Potentiometric titrations
11.5. Voltammetry
11.5.1. Basic principles and instrumentation
11.5.2. Voltammetric cells
11.5.3. Types of electrodes; indicator electrode, reference and auxiliary
11.5.4. Selective electrodes
11.5.5. Electrodes chemically modified
11.5.6. Linear scan voltammetry
11.5.7. Cyclic voltammetry
11.5.8. Anodic stripping voltammetry
11.5.9. Cathodic stripping voltammetry
11.5.10. Adsorptive stripping voltammetry

11.6. Analytical applications
Theoretical-practical matrix
Application of theoretical concepts in the practical laboratory matrix; performing calculations for preparing solutions, identifying the method of analysis according to the role of monographs and reference substances of Pharmacopoeias, GMP, GLP and ICH; treatment of experimental/graphic and statistical data and critical analysis.

Practical matrix
1. Qualitative and quantitative analysis of compounds- 12 hours
1.1.Impurities research in the purified water
1.2. Ion identify on polyelectrolytic solution
1.3. Identification and determination of paracetamol
1.4. Quality control of potassium hydroxide
2. Volumetric Analysis – 12 hours
2.1. Oxidation-reduction tritation- Determination of a concentrated solution of hydrogen peroxide
2.2. Complexing titration- Determination of magnesium sulfate to 20% Injectable
2.3 Precipitation tritations- chlorides by Mohr method
2.4- Acid-base tritations- Standard solutions of HCL
3. Treatment graphic and statistical analysis results
4. Potentiometric
4.1. Potentiometric determination of acetylsalicylic acid
4.2. Determination of fluoride concentration using selective electrode
4.3. Determination of chloride concentration using selective electrode

NAO

Equal