Analytical Chemistry In Pharmacy II

Laboratory techniques in Pharmacy, organic chemistry and inorganic chemistry

Expository theoretical classes using audiovisual and interactive means between student and teacher, using theoreticalpractical classes to reinforce the understanding of theoretical syllabus and its application in the laboratory matrix.
Two hours a week are available, with a pre-defined time or to be arranged with the student to clarify doubts.
Each student is also given the pedagogical support of all the material taught or the bibliographical references
recommended for the discipline, at the beginning of the semester.

The curricular unit of Analytical Chemistry in Pharmacy I aims to provide the student with an adequate knowledge of theoretical principles, qualitative and quantitative analysis, practical components and application of instrumental methods of analysis, indispensable to the performance of laboratory activities, with the main objective of applying new technologies to the field of Pharmacy, its validation, quality control, as well as in the area of Pharmaceutical Industry and research. Knowledge of GLP, GMP and ICH systems in the analysis of medicines in quality control, as well as compliance with quality specifications, identification, purity, dosage; role of Pharmacopoeia monographs and reference substances.

Theoretical matrix

1 UV-VISIBLE SPECTROSCOPY – 4h
1.1. Basic principles and instrumentation
1.2. The process of absorption and emission of ultraviolet-visible radiation
1.3. Photoelectric effect
1.4. Color
1.5. Electromagnetic Spectrum
1.6. Absorption and emission spectra of hydrogen
1.7. Electronic transitions
1.8. Ultraviolet-visible spectrophotometers
1.9. Beer-Lambert
1.10. Analytical applications
2. INFRARED SPECTROSCOPY  – 4 hours
2.1. Basic principles and instrumentation
2.2. The process of absorption of infrared radiation
2.3. Vibration modes
2.4. Applications of Infrared spectrum
2.5. Bands
2.6. Dispersive Espetrofotometros
2.7. Fourier transform Espetrofotometros
2.8. Sampling Techniques
2.8.1. Transmission techniques
2.8.2. Reflectance techniques
2.9. Analysis Infrared spectra
2.10. Analytical applications
3 SPECTROSCOPY OF NUCLEAR MAGNETIC resonance – 2 hours
3.1. Basic principles and instrumentation
3.2. Nuclear spin states
3.3. Energy absorption
3.4. The resonance phenomenon
3.5. Chemical shift and protection
3.6. Analytical Applications
4. SPECTROSCOPY ATOMIC ABSORPTION AND EMISSION – 4 hours
4.1. Basic principles and instrumentation
4.2. Chemical, physical and spectral interferences
4.3. Quantification
4.4. Atomic absorption spectroscopy flame
4.5. Atomic absorption spectroscopy with graphite furnace
4.6. Atomic emission spectroscopy Flame (ICP)
4.7. Analytical applications
5 Capillary Electrophoresis – 2 hours
5.1. Basic principles and instrumentation
5.2. Resolution and efficiency
5.3. electroosmosis
5.4. Capillary zone electrophoresis (CZE)
5.5. Isoelectric focusing (IEF)
5.6. Capillary gel electrophoresis (CGE)
5.7. “Isotachophoresis” capillary (ISCO)
5.8. Micellar electrokinetic capillary chromatography (MEKC)
5.9. analytical applications
6 ELEMENTAL ORGANIC ANALYSIS – 2 hours
6.1. Basics principles
6.2. Organic and inorganic compounds
6.3. Methods used in organic elemental analysis
6.4. Qualitative Analysis
6.5. Quantitative elemental analysis
6.6. Organic elemental analysis to obtain empirical formulas and molecular
7.CHROMATOGRAOHY – 8 hours
7.1. Basic principles
7.2. Liquid chromatography
7.2.1. Paper choromatography
7.2.2. Thin layer choromatography(TLC)
7.2.3. Column choromatography
7.2.3.1. Liquid-liquid choromatography
7.2.3.2. Solid-liquid choromatography
7.3. Gas chromatography
7.3.1. Instrumentation
7.3.2. Gas injection systems
7.3.3. Packed columns vs. capillary columns
7.3.4. Substrates and liquid phases
7.3.5. Gas-solid choromatography
7.3.6. Liquid gas choromatography
7.3.7. Adsorption choromatography
7.3.8. Partition choromatography
7.3.9. Types of detectors
7.4. High Performance Liquid Chromatography (HPLC) and Ultra Performance Liquid Chromatography
7.4.1. Iinstrumentation
7.4.2. Columns: types, applications and selection criteria
7.4.3. Types of interaction between the mobile phase and the stationary phase
7.4.3. Chemically bonded stationary phases
7.4.2. Normal phase chromatography and reverse phase
7.4. Analytical applications

Theoretical-practical matrix
Application of theoretical concepts in the practical laboratory matrix; performing calculations for preparing solutions, identifying the method of analysis according to the role of monographs and reference substances of Pharmacopoeias, GMP, GLP and ICH; treatment of experimental/graphic and statistical data and critical analysis.

Practical matrix

1. UV-Visible Spectroscopy – 12 hours

1.1. Copper sulfate absorption spectrum

1.2. Determination of nitrates and nitrites in water

1.3. Paracetamol absorption spectrum

1.4. Caffeine spectrophotometric assay

2. Chromatography – 21 hours

2.1. Determination of the amount of caffeine in beverages by HPLC

2.2. Simultaneous determination of paracetamol and ibuprofen by HPLC

2.3. Captopril dosage in tablets by HPLC

2.4. Simultaneous separation of estrogens by UPLC-FLR and their quantification

2.5. Fluoxetine separation by UPLC-MS and its quantification

 2.6. Simultaneous separation of estrone, estradiol, ethinyl estradiol and estriol by HPLC

3.- Solid phase extraction (SPE) of ethinylestradiol, HPLC analysis and recovery tests.

NAO

Equal