Biology II

Biology I  and Chemistry and Biochemistry I 

In order to achieve the objectives of the curricular unit, the teaching methodologies are based on:

1. In the presentation by the teacher of contents related to the theoretical aspects of this curricular unit;

2. In oriented bibliographic research on some topics of interest;

3. Practical laboratory work related to the subject;

4. In the elaboration of a practical work report.

The learning objectives of this Curricular Unit include the acquisition of knowledge of Microbial Molecular Genetics, on the flow of genetic information and its regulation in microorganisms, as well as the bases for potential applications. As the gene is used as a fundamental tool in molecular typing techniques of organisms, its study will be crucial, namely in the detection of pathogens, frauds and transgenics in food samples. It is intended that the student acquire basic skills in the area of the food industry, namely for quality control and food safety, food processing, production of substances of industrial food value and improvement of microbiological performance in manufacturing processes.

Module I

1. Methods of studying microbiology, 2. Microbiological control, 3. Factors that affect the growth of microorganisms.

Module II

1. Nucleic acids and DNA replication; 2. Transcription and protein synthesis; 3. Regulation of gene expression; 4. Genetic variability; 5. DNA extraction and visualization protocols; 6. Typification of organisms by molecular methods; 7. Applications of Molecular Genetics in the food field.

• - Testes Teórico-Práticos e Relatórios - 100.0%

• - Prova escrita Teórico-Prática - 100.0%

NAO

Azevedo, C., Sunkel, C. Biologia Celular e Molecular. 5ª ed, Lidel – Edições Técnicas, Lisboa, 629 p., 2012.

Fialho, A.M., Arraiano, C.M. O mundo do RNA. Novos desafios e perspectivas futuras. 1ª. ed., Lidel, Lisboa, 296p., 2007.

Gardner, Simmons, Snustad. Principles of Genetics, 8th ed., Wiley India Pvt. Limited, 740p. 2006.

Hartl, D., Ruvolo, Maryellen. Genetics. Analysis of Genes and Genomes. 8ª ed., Jones and Bartlett Learnig, Sudbury, 2011.

Lodish, H., Berk, A., Kaiser, C., Krieger, M., Bretscher; A., Ploegh, H., Amon, A., Scott, M. Molecular Cell Biology. 7ª ed. W.H. Freeman and Company. Nova Iorque. 2012.

Madigan, M.T., Martinko, J.M., Bender, K., Buckley, D.P., Stahl, D.A. Brock Biology of Microorganisms. 13ª ed., Benjamin Cummings, Pearson, 2012.

Nicholl, D.S.T. An Introduction to Genetic Engineering. Cambridge University Press, 1994.

Sadava, D.E., Hillis, D.M., Heller, H.C., Berenbaum, M. Life: The Science of Biology. 10ª ed., Publisher, W.H Freeman, Massachussets, 2014.

Scharer-Zublin, E.V. Os Genes e a Alimentação. Fondation Alimentarium. Nestlé, 2003.

Smith, C.A., Wood, E.J. Molecular Biology and Biotechnology. 1ª ed., Chapman & Hall, Londres, 1991.

Willey, J.M., Sherwood, L. e Woolverton, C. Prescott’s Microbiology. 9ª ed., McGraw-Hill. 2013.