Cell Metabolism and Bioenergetics

Knowledge of the contents of:
– Chemistry and Biochemistry
– Biology
– Cell Physiology

Theoretical teaching is based on oral presentation supported by multimedia presentations. Students will be given the opportunity to discuss, when pertinent to the contents to be taught in class, and the discussions are moderated by the teacher. In the general presentation of the subjects in the theoretical-practical classes are presented examples of application of the subject, where appropriate.
Discussion of the basis of experimental work (at the beginning of the experimental lectures), results achieved obtained and their interpretation (at the end of the lectures). In the theoretical-practical component, a discussion space will be provided on the topics under study and their application in other contexts, and students will be encouraged to proceed to a previous reading of the recommended bibliography.
Theoretical-practical component, which includes a laboratory component by module and discussion of scientific articles.

1. Compare and understand different cellular anabolic and catabolic pathways in living beings belonging to the three Domains of Life.
2. Understand the oxidation pathways to achieve chemical energy.
3. Integrate the various cellular metabolic pathways.

1. Cell Bioenergetics
1.1. Bioenergetics and Introduction to Metabolism.
1.2. Metabolism of carbohydrates: Glycolysis and Neoglucogenesis; Krebs Cycle;Respiratory complexes, electronic transport and mitochondrial oxidative phosphorylation; Pentoses-phosphate pathway; Route of uronic acid; Biosynthesis and degradation of glycogen.
1.3. Lipid Metabolism: Catabolism of Fatty Acids;
1.4. Catabolism of amino acids.

2. Non-Oxidative Metabolic Pathways
2.1. Fatty acid anabolism; Biosynthesis of triglycerides, phosphoglycerides and sphingolipids; Biosynthesis of cholesterol and its regulation: Biosynthesis of cholesterol derivatives: bile acids and steroid hormones; Transport of lipids: lipoprotein metabolism.
2.2. Metabolism of nitrogen compounds: Biosynthesis of amino acids; Biosynthesis and catabolism of purines: regulation; Biosynthesis and catabolism of pyrimidines.Biosynthesis of ribo- and deoxyribonucleotides.
2.3. Integration of Metabolism.

• - Avaliação por Frequência - testes - 55.0%
• - Avaliação por Frequência - apresentação de artigo - 10.0%
• - Avaliação por Frequência - relatórios - 35.0%

NAO

Bibliografia de base
LODISH, H., BERK, A., KAISER, C.A., KRIEGER, M., BRETSCHER, A., PLOEGH, H., AMON, A. & MARTIN, K.C. “Molecular Cell Biology” 8th Edition, W.H. Freeman & Co (New York, USA), 2016. ISBN: 978-1464183393
BERG, J.M., TYMOCZKO, J.L., GATTO, G.J., STRYER, L.,.”Biochemistry” 8th Edition W.H. Freeman & Co (New York, USA), 2015. ISBN: 978-1464126109
NELSON D. L., COX, M.M. “Lehninger: Principles of Biochemistry” 6th Edition W H Freeman & Co (USA), 2012. ISBN: 978-1429234146

– Bibliografia complementar
COOPER, G. & HAUSMANN, R.E. “The Cell – a Molecular Approach” 5th Edition, Sinauer Associates Inc. Publisheres, USA, 2006. ISBN: 978-0878933006
METZLER, D. E.; METZLER, C. M.; SAUKE, D. J. “Biochemistry: The Chemical Reactions of Living Cells” 2nd Edition. Vol 1. Academic Press, 2003. ISBN: 978-0124925410
METZLER, D. E.; METZLER, C. M.; SAUKE, D. J. (2003) “Biochemistry: The Chemical Reactions of Living Cells” 2nd Edition, Vol 2. Academic Press, 2003. ISBN: 978-0124925