Base Knowledge
Clinical Laboratory Microbiology I
Teaching Methodologies
The theoretical component, although predominantly expository, in order to provide the information inherent to the topics to be addressed, also intends to explore, whenever appropriate, the intervention of students and the discussion of topics, or clinical cases, as long as they are relevant to the themes to be addressed to develop. In the Laboratory-Practice component, the individual application of the planned laboratory procedures will be predominant. The observed results will be discussed and evaluated in groups.
Learning Results
It is intended that the student acquires knowledge about:
• Microbiological evaluation of biological samples including collection, packaging and laboratory processing; Basic concepts of fungi and pathogenesis of fungal diseases; Classification of mycoses and respective laboratory diagnosis; Basic concepts of parasites and pathogenesis of parasitic diseases; Laboratory diagnosis of parasitic diseases.
It is also intended that the student is able to:
• Carry out the microbiological analysis of several biological samples taking into account the identification and characterization of susceptibility of the bacterial agent (s) responsible for the infection process; Carry out the laboratory examination of biological samples for the study of relevant clinical mycoses; Carry out the laboratory examination of biological samples to study relevant parasites.
The student must also acquire skills to:
• Choose the laboratory procedures that, according to each situation and available resources, can best contribute to the intended objective; Recognize the advantages and disadvantages inherent to the procedures applied; Integrate into the laboratory routine of a Microbiology Laboratory, a fact that will allow the student to attend the Microbiology internship provided for in their training.
Program
Theoretical Program
1-Microbiological study of biological products.
Collection, storage and laboratory processing for the microbiological analysis of several biological products:
A. Urocultures,
B. Superficial and deep exudates,
C. Other organic liquids,
D. Blood culture,
E. Sputum (and BAAR),
F. Stool
G. Other types of samples…
2- Clinical Mycology
A. Pathogenesis of Fungal Diseases,
B. Basic concepts about fungi of clinical interest.
C. Classification of mycoses and respective laboratory diagnosis: cutaneous, systemic, opportunistic mycoses.
D. Antifungals and resistance phenomena
3- Clinical parasitology
A. Pathogenesis of parasitic diseases,
B. Introduction to clinical parasitology and laboratory diagnosis of parasitosis: intestinal and urogenital, blood and tissues.
C. Nematodes, trematodes, worms and arthropods
D. Antiparasites drugs
Laboratory Practice Program
1-Microbiological study of biological samples: urine, exudates, CSF, blood culture, sputum, stool – cultural examination and differentiation of the causative agent of infection
2-Laboratory diagnosis of mycoses: cutaneous, systemic and opportunistic – microscopic observation, cultural examination and differentiation of the causative agent of infection.
3-Laboratory diagnosis of parasitosis: intestinal and blood, performing concentration technique (Ritchie), microscopic observation and tests for antigen research
Curricular Unit Teachers
Internship(s)
NAO
Bibliography
Primary references
Murray, PR; Rosenthal, KS; Pfaller, MA. (2010) Microbiologia Médica. 6ª ed. MOSBY ELSEVIER, Rio de Janeiro
Ferreira, WFC, Sousa, JCF, Lima, N (2010). Microbiologia, ed. LIDEL, Lisboa
Barroso H, Taveira N, Meliço-Silvestre A (2014). Microbiologia Médica (1 e 2), ed. LIDEL, Lisboa
Madigan, MT Martinho, JM, Dunlap, PV, Clark, DP (2009). Brock – Biology of microorganisms, 12th ed, Pearson International Edition, Pearsons Benjamin Cummings, USA
Krasner, RI (2010). The Microbial Challenge – Science, Disease and Public Health, 2nd ed, Jones and Bartlet Publishers, UK
G. J. Tortora, B. R. Funke, C. L. Case. (2000) Microbiologia, 6ª ed. Artmed Editora. Porto Alegre. Brasil.