Culture of Plant Cells

Biology

To achieve the objectives of the curricular unit and confer the skills expected of students, the teaching and learning process is based on the following teaching methodologies:
a) In teaching theoretical classes;
b) Teaching practical classes in the laboratory;
c) Carrying out practical work in the field (collection of plant material), in the laboratory (cell culture) and in the nursery (acclimatization of seedlings obtained by in vitro propagation);
d) In bibliographical research and analysis of articles and reports on the contents of the UC.
To better consolidate the concepts and methods used in classes (in the laboratory or in a nursery), students are accompanied weekly in tutorial classes.
Students are required to attend classes. The minimum attendance value is 75% of the number of classes taught.

The learning objective of this curricular unit is to promote, in the student who successfully completes it, the following skills identified below.
1. Know the techniques applied in plant cell cultures and their applications in the improvement and conservation of genetic resources.
2. Identifies methods for medium and long-term conservation. Know the techniques applied in plant cell cultures and their applications in increasing genetic variability and in the conservation and evaluation of plant germplasm.

The acquisition of skills in Module 1 is achieved through the following learning outcomes:
a). Identifies the influence of plant material and physical factors on the growth and development of crops.
b). Identifies the different types of vegetative multiplication in vitro.
c). Identifies the various development stages and their specific requirements.
The acquisition of skills in Module 2 is obtained through the following learning outcomes:
a). Identifies the different methods of preserving plant cultures in vitro.
b). Identifies the different methods for evaluating the efficiency of in vitro plant culture conservation techniques.

The program contents to achieve the two learning objectives described are divided into two learning modules:
Module 1: Plant cell culture, its applications in plant improvement and conservation of genetic resources.
Module 2: Plant cell culture, practical application and case studies.

• - O Módulo 1 ponderação de 65 %. Módulo 1: 1) testes (75%); 2) trabalhos práticos (10%); 3) apresentação artigo (15%). - 65.0%
• - Módulo 2 ponderação de 35 %. 1) testes (85%); 2) avaliação trabalhos práticos (15%). - 35.0%

NAO

CANHOTO, J.M. – Biotecnologia vegetal, da clonagem de plantas à transformação genética. Imprensa da Universidade de Coimbra. 2010. ISBN 978-989-26-0065-9.
ENGELMANN, F. – Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell.Dev.Biol.Plant. DOI 10.1007/s11627-010-9327-2. Vol: 47, 2010, 5-16.
GEORGE, E.F., DE KLERK, G.J. The components of plant tissue culture media I: macro and micro-nutrients. In Plant propagation by tissue culture, 3rd ed. (eds. George, E.F., Hall, M.A. & De Klerk, G.J.), Springer, Dordrecht. ISBN 978-1-4020-5004-6, 2008, pp. 65-114.
OZUDOGRU, E.A., PREVIATI, A., LAMBARDI, M. In Vitro Conservation and Cryopreservation of Ornamental Plants. In Protocols for in Vitro Propagation of Ornamental Plants, Methods in Molecular Biology, Jain, S.M. & Ochatt, S.J. (eds.), vol. 589, DOI 10.1007/978-1-60327-114-1_28, © Humana Press, LLC, 2010, pp. 303-324.
PREECE, J. – Stock plant physiological factors affecting growth and morphogenesis. In: Plant propagation by tissue culture, 3rd ed., George, E.F., Hall, M.A., De Klerk, G.J. (Eds.), Springer, Dordrecht. ISBN 978-1-40205004-6, 2008, pp. 403-422.
PUROHIT, S.D.-.Introduction to plant cell tissue and organ culture. PHI Learning Private Limited. Delhi, 2012, ISBN 978-81-203-4677-2.