Microbiology I

Knowledge of Microbiology in several areas of health and environment has enormous importance. In this context it
is essential for the students recognize the morphological and physiological characteristics of different groups of
microorganisms (bacteria, virus, fungi and parasites). The student should also understand the mechanisms of
action of several antimicrobial agents. It is also aimed the developing of basic skills related to handling biological
material, complying the basic safety rules in microbiology laboratory and to distinguish the mainly important
methodologies involved in the control and prevention.

History of Microbiology . Organization and function of the eukaryotic and procriotic cell; Virology: virus and prions,
lytic and lysogenic cycle; Bacteriophages; Bacteriology: structure and morphology. Bacteria with clinical
importance: Gram + cocci, Gram – rods, acid-alcohol resistant rods, others. Interaction between microorganisms
and higher beings. Normal flora, transient, opportunistic and pathogenic; Microalgae and cyanobacteria; Mycology:
classification and potentialities of fungi; Parasitology: morphological characteristics, life cycles and routes of
transmission. Human parasites; Control of microbial growth: physical and chemical methods.
THEORETICAL-PRATICAL AND PRACTICAL PROGRAM
Laboratory of Microbiology: biosafety; Optical microscope; Bacterial staining: direct slide and dye slide (Gram
technique); Manipulation in assepsia: sterilization, preparation of material/culture media; Culture of microrganisms;
Biochemical characterization – galleries identification.

NAO

1. Brock Biology of Microorganisms (14th Edition) 2012. M. T. Mandigan; J. M. Martinko; K. S. Bender; D. H.
Buckley; D. A. Stahl. Pearson Education (US).
3. Microbiologia – Volume I, 1998. W. F. Canas Ferreira, J. C. de Sousa. Lídel – Edições Técnicas. Lisboa. Portugal.
4. Microbiologia – Volume II, 2000. W. F. Canas Ferreira, J. C. de Sousa. Lídel – Edições Técnicas. Lisboa. Portugal
5. Microbiologia – Conceitos e aplicações, 1996. M. J. Pelczar, Jr.; E. C. S. Chan; N. R. Krieg. Makron Books. São
Paulo. Brasil, Inc.
8. Microbiologia Médica e Imunologia 4ª. Ed. 1998. W. Levinson, E. Jawetz. Artes Médicas. Porto Alegre. Brasil.
9. Manual of Environmental Microbiology, 2nd Ed., 2002. C. J. Hurst, R. L. Crawford, G. R. Knudsen, M. J. McInerney,
L. D. Stetzenbach. American Society for Microbiology, Washinton, DC.
10. Biology of Microorganisms, 8th Ed., 1996. T. D. Brock; M. T. Mandigan; J. M. Martinko; J. Parker. Prentice Hall,
Englewood Cliffs, New Jersey